Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.
Source:
Balkan Journal of Medical Genetics, 2000, 3, 3, 19-22
TY - JOUR
AU - Potparević, Biljana
AU - Bajić, V
PY - 2000
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/277
AB - Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.
PB - Macedonian Academy of Sciences and Arts
T2 - Balkan Journal of Medical Genetics
T1 - Premature centromere separation syndromes: A manifestation of genome instability?
VL - 3
IS - 3
SP - 19
EP - 22
UR - https://hdl.handle.net/21.15107/rcub_farfar_277
ER -
@article{
author = "Potparević, Biljana and Bajić, V",
year = "2000",
abstract = "Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.",
publisher = "Macedonian Academy of Sciences and Arts",
journal = "Balkan Journal of Medical Genetics",
title = "Premature centromere separation syndromes: A manifestation of genome instability?",
volume = "3",
number = "3",
pages = "19-22",
url = "https://hdl.handle.net/21.15107/rcub_farfar_277"
}
Potparević, B.,& Bajić, V.. (2000). Premature centromere separation syndromes: A manifestation of genome instability?. in Balkan Journal of Medical Genetics
Macedonian Academy of Sciences and Arts., 3(3), 19-22.
https://hdl.handle.net/21.15107/rcub_farfar_277
Potparević B, Bajić V. Premature centromere separation syndromes: A manifestation of genome instability?. in Balkan Journal of Medical Genetics. 2000;3(3):19-22.
https://hdl.handle.net/21.15107/rcub_farfar_277 .
Potparević, Biljana, Bajić, V, "Premature centromere separation syndromes: A manifestation of genome instability?" in Balkan Journal of Medical Genetics, 3, no. 3 (2000):19-22,
https://hdl.handle.net/21.15107/rcub_farfar_277 .
