Premature centromere separation syndromes: A manifestation of genome instability?
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Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.
Извор:Balkan Journal of Medical Genetics, 2000, 3, 3, 19-22
- Macedonian Academy of Sciences and Arts