A new method for the determination of xanthine oxidase activity, based on the oxidation of 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) by use of uricase and peroxidase, is described. The absorbance increase of the oxidized form of ABTS, measured after 10 min at 410 nm is proportional to xanthine oxidase activity. The method is sensitive, precise (CV below 8.3%), and linear up to 20 U/l. The analytical recovery of the ABTS-method was quantitative. Comparison with the UV and colorimetric NBT-method gave good correlation (r ≥ 0.984). Reference values for serum xanthine oxidase activities determined with the new ABTS-method on 83 healthy persons are 0 to 1.20 U/l.
TY - JOUR
AU - Majkić-Singh, Nada
AU - Bogavac, L
AU - Kalimanovska, Vesna
AU - Jelić, Z
AU - Spasić, S
PY - 1987
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/28
AB - A new method for the determination of xanthine oxidase activity, based on the oxidation of 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) by use of uricase and peroxidase, is described. The absorbance increase of the oxidized form of ABTS, measured after 10 min at 410 nm is proportional to xanthine oxidase activity. The method is sensitive, precise (CV below 8.3%), and linear up to 20 U/l. The analytical recovery of the ABTS-method was quantitative. Comparison with the UV and colorimetric NBT-method gave good correlation (r ≥ 0.984). Reference values for serum xanthine oxidase activities determined with the new ABTS-method on 83 healthy persons are 0 to 1.20 U/l.
T2 - Clinica Chimica Acta
T1 - Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen
VL - 162
IS - 1
SP - 29
EP - 36
DO - 10.1016/0009-8981(87)90229-4
ER -
@article{
author = "Majkić-Singh, Nada and Bogavac, L and Kalimanovska, Vesna and Jelić, Z and Spasić, S",
year = "1987",
abstract = "A new method for the determination of xanthine oxidase activity, based on the oxidation of 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) by use of uricase and peroxidase, is described. The absorbance increase of the oxidized form of ABTS, measured after 10 min at 410 nm is proportional to xanthine oxidase activity. The method is sensitive, precise (CV below 8.3%), and linear up to 20 U/l. The analytical recovery of the ABTS-method was quantitative. Comparison with the UV and colorimetric NBT-method gave good correlation (r ≥ 0.984). Reference values for serum xanthine oxidase activities determined with the new ABTS-method on 83 healthy persons are 0 to 1.20 U/l.",
journal = "Clinica Chimica Acta",
title = "Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen",
volume = "162",
number = "1",
pages = "29-36",
doi = "10.1016/0009-8981(87)90229-4"
}
Majkić-Singh, N., Bogavac, L., Kalimanovska, V., Jelić, Z.,& Spasić, S.. (1987). Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen. in Clinica Chimica Acta, 162(1), 29-36.
https://doi.org/10.1016/0009-8981(87)90229-4
Majkić-Singh N, Bogavac L, Kalimanovska V, Jelić Z, Spasić S. Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen. in Clinica Chimica Acta. 1987;162(1):29-36.
doi:10.1016/0009-8981(87)90229-4 .
Majkić-Singh, Nada, Bogavac, L, Kalimanovska, Vesna, Jelić, Z, Spasić, S, "Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen" in Clinica Chimica Acta, 162, no. 1 (1987):29-36,
https://doi.org/10.1016/0009-8981(87)90229-4 . .
