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Spectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogen

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1987
Authors
Majkić-Singh, Nada
Bogavac, L
Kalimanovska, Vesna
Jelić, Z
Spasić, S
Article (Published version)
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Abstract
A new method for the determination of xanthine oxidase activity, based on the oxidation of 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) by use of uricase and peroxidase, is described. The absorbance increase of the oxidized form of ABTS, measured after 10 min at 410 nm is proportional to xanthine oxidase activity. The method is sensitive, precise (CV below 8.3%), and linear up to 20 U/l. The analytical recovery of the ABTS-method was quantitative. Comparison with the UV and colorimetric NBT-method gave good correlation (r ≥ 0.984). Reference values for serum xanthine oxidase activities determined with the new ABTS-method on 83 healthy persons are 0 to 1.20 U/l.
Keywords:
ABTS / Evaluation / Peroxidase / Reference value / Spectrophotometric assay / Xanthine oxidase
Source:
Clinica Chimica Acta, 1987, 162, 1, 29-36

DOI: 10.1016/0009-8981(87)90229-4

ISSN: 0009-8981

Scopus: 2-s2.0-0023125683
[ Google Scholar ]
32
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/28
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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