Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties
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2017
Authors
Pavlović, Dragana R.Veljković, Milica
Stojanović, Nikola M.

Gocmanac-Ignjatović, Marija
Mihailov-Krstev, Tatjana
Branković, Suzana
Sokolović, Dušan
Marčetić, Mirjana

Radulović, Niko

Radenković, Mirjana
Article (Published version)

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ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activi...ty. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.
Keywords:
Allium ursinum / enteropathogenic strains / qNMR of alk(en)yl cysteine sulphoxides / rat ileum / spasmolytic activitySource:
Journal of Pharmacy and Pharmacology, 2017, 69, 9, 1208-1218Publisher:
- Wiley, Hoboken
Funding / projects:
DOI: 10.1111/jphp.12746
ISSN: 0022-3573
PubMed: 28543032
WoS: 000407083000014
Scopus: 2-s2.0-85019619634
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Institution/Community
PharmacyTY - JOUR AU - Pavlović, Dragana R. AU - Veljković, Milica AU - Stojanović, Nikola M. AU - Gocmanac-Ignjatović, Marija AU - Mihailov-Krstev, Tatjana AU - Branković, Suzana AU - Sokolović, Dušan AU - Marčetić, Mirjana AU - Radulović, Niko AU - Radenković, Mirjana PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2802 AB - ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances. PB - Wiley, Hoboken T2 - Journal of Pharmacy and Pharmacology T1 - Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties VL - 69 IS - 9 SP - 1208 EP - 1218 DO - 10.1111/jphp.12746 ER -
@article{ author = "Pavlović, Dragana R. and Veljković, Milica and Stojanović, Nikola M. and Gocmanac-Ignjatović, Marija and Mihailov-Krstev, Tatjana and Branković, Suzana and Sokolović, Dušan and Marčetić, Mirjana and Radulović, Niko and Radenković, Mirjana", year = "2017", abstract = "ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.", publisher = "Wiley, Hoboken", journal = "Journal of Pharmacy and Pharmacology", title = "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties", volume = "69", number = "9", pages = "1208-1218", doi = "10.1111/jphp.12746" }
Pavlović, D. R., Veljković, M., Stojanović, N. M., Gocmanac-Ignjatović, M., Mihailov-Krstev, T., Branković, S., Sokolović, D., Marčetić, M., Radulović, N.,& Radenković, M.. (2017). Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology Wiley, Hoboken., 69(9), 1208-1218. https://doi.org/10.1111/jphp.12746
Pavlović DR, Veljković M, Stojanović NM, Gocmanac-Ignjatović M, Mihailov-Krstev T, Branković S, Sokolović D, Marčetić M, Radulović N, Radenković M. Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology. 2017;69(9):1208-1218. doi:10.1111/jphp.12746 .
Pavlović, Dragana R., Veljković, Milica, Stojanović, Nikola M., Gocmanac-Ignjatović, Marija, Mihailov-Krstev, Tatjana, Branković, Suzana, Sokolović, Dušan, Marčetić, Mirjana, Radulović, Niko, Radenković, Mirjana, "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties" in Journal of Pharmacy and Pharmacology, 69, no. 9 (2017):1208-1218, https://doi.org/10.1111/jphp.12746 . .