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Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension

Authorized Users Only
2017
Authors
Toljić, Mina
Egić, Amira
Munjas, Jelena
Orlić, Nataša Karadzov
Milovanović, Zagorka
Radenković, Aleksandra
Vuceljić, Jovana
Joksić, Ivana
Article (Published version)
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Abstract
We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only du...ring pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.

Keywords:
Gestational diabetes mellitus / Gestational arterial hypertension / Oxidative stress / Micronuclei / Cytokinesis-block micronucleus cytome assay / Malondialdehyde / 8-Hydroxy-2'-deoxyguanosine / Methyldopa
Source:
Research in Microbiology, 2017, 71, 55-62
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Projects:
  • Radiosensitivity of human genome (RS-173046)

DOI: 10.1016/j.reprotox.2017.04.002

ISSN: 0890-6238

PubMed: 28400286

WoS: 000406736600006

Scopus: 2-s2.0-85018649006
[ Google Scholar ]
16
13
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2808
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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