Increased oxidative stress and cytokinesis-block micronucleus cytome assay parameters in pregnant women with gestational diabetes mellitus and gestational arterial hypertension
Authorized Users Only
2017
Authors
Toljić, MinaEgić, Amira
Munjas, Jelena

Orlić, Nataša Karadzov
Milovanović, Zagorka
Radenković, Aleksandra
Vuceljić, Jovana
Joksić, Ivana
Article (Published version)

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Show full item recordAbstract
We investigated whether gestational diabetes mellitus (GDM) and gestational arterial hypertension (GH) are associated with increased oxidative stress and DNA damage. Study included 3 groups of pregnant women (GDM, GH and control). DNA damage biomarkers (micronuclei MNi, nucleoplasmic bridges NPBs and nuclear buds NBUDs) were assessed by cytokinesis-block micronucleus cytome assay. Oxidative stress levels were evaluated by analyzing malondialdehyde equivalents (TBARS) and 8-hydroxy-2'deoxyguanosine (8-OHdG). Genotoxic effect of methyldopa, drug used to treat GH, was evaluated in in vitro experiment. TBARS levels, MNi, NPBs and NBUDs frequencies were significantly increased in both GDM and GH group. Concentrations of 8-OHdG were significantly higher in GDM than in other groups. Since methyldopa did not affect MNi, NPBs and NBUDs frequencies, nor TBARS and 8-OHdG levels, we concluded that methyldopa has no genotoxic effect. Thus, even when hyperglycemia or hypertension are present only du...ring pregnancy they induce oxidative stress, DNA damage and chromosomal aberrations.
Keywords:
Gestational diabetes mellitus / Gestational arterial hypertension / Oxidative stress / Micronuclei / Cytokinesis-block micronucleus cytome assay / Malondialdehyde / 8-Hydroxy-2'-deoxyguanosine / MethyldopaSource:
Research in Microbiology, 2017, 71, 55-62Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Projects:
- Radiosensitivity of human genome (RS-173046)
DOI: 10.1016/j.reprotox.2017.04.002
ISSN: 0890-6238
PubMed: 28400286