Alterations of HDL Particles in Children with End-Stage Renal Disease

2017
Authors
Stefanović, Aleksandra
Ristovski-Kornić, Danijela
Kotur-Stevuljević, Jelena

Spasojević-Kalimanovska, Vesna

Vekić, Jelena

Miljković, Milica

Paripović, Dušan

Peco-Antić, Amira
Jelić-Ivanović, Zorana

Zeljković, Aleksandra

Article (Published version)

Metadata
Show full item recordAbstract
Background: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. Methods: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). Results: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses wh...en compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. Conclusions: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality arenot routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information.
Keywords:
chronic kidney disease / HDL particles / myeloperoxidaseSource:
Journal of Medical Biochemistry, 2017, 36, 4, 358-365Publisher:
- Društvo medicinskih biohemičara Srbije, Beograd i Versita
Projects:
DOI: 10.1515/jomb-2017-0019
ISSN: 1452-8258
PubMed: 30581333