Alterations of HDL Particles in Children with End-Stage Renal Disease
2017
Аутори
Stefanović, AleksandraRistovski-Kornić, Danijela
Kotur-Stevuljević, Jelena
Spasojević-Kalimanovska, Vesna
Vekić, Jelena
Miljković, Milica
Paripović, Dušan
Peco-Antić, Amira
Jelić-Ivanović, Zorana
Zeljković, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. Methods: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). Results: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses wh...en compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. Conclusions: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality arenot routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information.
Кључне речи:
chronic kidney disease / HDL particles / myeloperoxidaseИзвор:
Journal of Medical Biochemistry, 2017, 36, 4, 358-365Издавач:
- Društvo medicinskih biohemičara Srbije, Beograd i Versita
Финансирање / пројекти:
DOI: 10.1515/jomb-2017-0019
ISSN: 1452-8258
PubMed: 30581333
WoS: 000408878600009
Scopus: 2-s2.0-85029396541
Институција/група
PharmacyTY - JOUR AU - Stefanović, Aleksandra AU - Ristovski-Kornić, Danijela AU - Kotur-Stevuljević, Jelena AU - Spasojević-Kalimanovska, Vesna AU - Vekić, Jelena AU - Miljković, Milica AU - Paripović, Dušan AU - Peco-Antić, Amira AU - Jelić-Ivanović, Zorana AU - Zeljković, Aleksandra PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2815 AB - Background: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. Methods: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). Results: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses when compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. Conclusions: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality arenot routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information. PB - Društvo medicinskih biohemičara Srbije, Beograd i Versita T2 - Journal of Medical Biochemistry T1 - Alterations of HDL Particles in Children with End-Stage Renal Disease VL - 36 IS - 4 SP - 358 EP - 365 DO - 10.1515/jomb-2017-0019 ER -
@article{ author = "Stefanović, Aleksandra and Ristovski-Kornić, Danijela and Kotur-Stevuljević, Jelena and Spasojević-Kalimanovska, Vesna and Vekić, Jelena and Miljković, Milica and Paripović, Dušan and Peco-Antić, Amira and Jelić-Ivanović, Zorana and Zeljković, Aleksandra", year = "2017", abstract = "Background: Unfavorable lipid profile presents one of most important risk factor for cardiovascular disease in renal pathology. Myeloperoxidase (MPO) as enzyme which oxidizes lipoproteins and paraoxonase1 (PON1) as anti-oxidative enzyme have been involved in pathogenesis of cardiovascular disease. In the present study we sought to assess oxidative stress status, lipoprotein subclasses distribution as well as functionality of high density lipoprotein (HDL) trough MPO/PON1 ratio in children with chronic kidney disease (CKD) and children after renal transplantation. Methods: PON1 activity and oxidative stress parameters were measured spectrophotometrically, while MPO concentration was determined using immunoassay. Separation of lipoprotein subclasses was performed by vertical gradient gel electrophoresis in 19 children with different stage of CKD and 19 post-transplantation patients (PT). Results: CKD patients had increased MPO/PON1 ratio and higher prevalence of smaller HDL subclasses when compared to PT subjects. Also, there was a significant positive correlation between MPO level and MPO/PON1 ratio with relative proportion of smaller HDL subclasses. Conclusions: Children with CKD have impaired HDL distribution that is improved after kidney transplantation. Since that measurement of HDL distribution and functionality arenot routinely available, MPO/PON1 ratio may be useful marker that could provide necessary information.", publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita", journal = "Journal of Medical Biochemistry", title = "Alterations of HDL Particles in Children with End-Stage Renal Disease", volume = "36", number = "4", pages = "358-365", doi = "10.1515/jomb-2017-0019" }
Stefanović, A., Ristovski-Kornić, D., Kotur-Stevuljević, J., Spasojević-Kalimanovska, V., Vekić, J., Miljković, M., Paripović, D., Peco-Antić, A., Jelić-Ivanović, Z.,& Zeljković, A.. (2017). Alterations of HDL Particles in Children with End-Stage Renal Disease. in Journal of Medical Biochemistry Društvo medicinskih biohemičara Srbije, Beograd i Versita., 36(4), 358-365. https://doi.org/10.1515/jomb-2017-0019
Stefanović A, Ristovski-Kornić D, Kotur-Stevuljević J, Spasojević-Kalimanovska V, Vekić J, Miljković M, Paripović D, Peco-Antić A, Jelić-Ivanović Z, Zeljković A. Alterations of HDL Particles in Children with End-Stage Renal Disease. in Journal of Medical Biochemistry. 2017;36(4):358-365. doi:10.1515/jomb-2017-0019 .
Stefanović, Aleksandra, Ristovski-Kornić, Danijela, Kotur-Stevuljević, Jelena, Spasojević-Kalimanovska, Vesna, Vekić, Jelena, Miljković, Milica, Paripović, Dušan, Peco-Antić, Amira, Jelić-Ivanović, Zorana, Zeljković, Aleksandra, "Alterations of HDL Particles in Children with End-Stage Renal Disease" in Journal of Medical Biochemistry, 36, no. 4 (2017):358-365, https://doi.org/10.1515/jomb-2017-0019 . .