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dc.creatorSavić, Vedrana
dc.creatorTodosijević, Marija N.
dc.creatorIlić, Tanja
dc.creatorLukić, Milica
dc.creatorMitsou, Evgenia
dc.creatorPapadimitriou, Vassiliki
dc.creatorAvramiotis, Spyridon
dc.creatorMarković, Bojan
dc.creatorCekić, Nebojša
dc.creatorSavić, Snežana
dc.date.accessioned2019-09-02T11:58:04Z
dc.date.available2019-09-02T11:58:04Z
dc.date.issued2017
dc.identifier.issn0378-5173
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2823
dc.description.abstractIn order to improve skin penetration of tacrolimus we aimed to develop potentially non-irritant, lecithin-based microemulsions containing ethanol, isopropanol and/or propylene glycol as cosurfactants, varying caprylic/capric triglycerides and propylene glycol monocaprylate as oil phase. The influence of excipients on the size of microemulsion region in pseudo-ternary phase diagrams and their ability to form different types of microemulsions was evaluated. The comprehensive physicochemical characterization of microemulsions and the evaluation of their structure was performed, while the localization of tacrolimus in microemulsions was further investigated using electron paramagnetic resonance spectroscopy. Moreover, stability studies proved no change in tacrolimus content during one year of storage at room temperature. In addition, in vivo skin performance indicated no skin irritation potential of blank microemulsions, whereas in vitro release testing using Franz diffusion cells showed superior release rate of tacrolimus from microemulsions (0.98 +/- 0.10 and 0.92 +/- 0.11 mu g/cm(2)/h for two bicontinuous and 1.00 +/- 0.24 mu g/cm(2)/h for oil-in-water microemulsion) compared to referent Protopic ointment (0.15 +/- 0.08 mu g/cm(2)/h). Furthermore, ex vivo penetration assessed through porcine ear skin using tape stripping, confirmed superiority of two microemulsions related to the reference, implying developed microemulsions as promising carriers for dermal delivery of tacrolimus.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/34031/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Pharmaceutics
dc.subjectLecithin-based microemulsionsen
dc.subjectTacrolimusen
dc.subjectPropylene glycol monocaprylateen
dc.subjectSkin irritation potentialen
dc.subjectElectron paramagnetic resonance spectroscopyen
dc.subjectIn vitro releaseen
dc.subjectTape strippingen
dc.titleTacrolimus loaded biocompatible lecithin-based microemulsions with improved skin penetration: Structure characterization and in vitro/in vivo performancesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЦекић, Небојша; Илић, Тања; Aврамиотис, Спyридон; Марковић, Бојан; Митсоу, Евгениа; Савић, Ведрана; Тодосијевић, Марија Н.; Пападимитриоу, Вассилики; Савић, Снежана; Лукић, Милица;
dc.citation.volume529
dc.citation.issue1-2
dc.citation.spage491
dc.citation.epage505
dc.citation.other529(1-2): 491-505
dc.citation.rankM21
dc.identifier.wos000408009200046
dc.identifier.doi10.1016/j.ijpharm.2017.07.036
dc.identifier.pmid28711641
dc.identifier.scopus2-s2.0-85024906743
dc.identifier.rcubconv_3925
dc.type.versionpublishedVersion


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