Aim: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. Materials & methods: Separation was performed on Acquity BEH amide column (2.1 x 100 mm, 1.7 mu m). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized. Results: The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated. Conclusion: The proposed method was utilized for monitoring of olopatadine in human tear.
TY - JOUR
AU - Maksić, Jelena
AU - Stajić, Ana
AU - Knežević, Miroslav
AU - Dacić-Krnjaja, Bojana
AU - Jančić-Stojanović, Biljana
AU - Medenica, Mirjana
PY - 2017
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2860
AB - Aim: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. Materials & methods: Separation was performed on Acquity BEH amide column (2.1 x 100 mm, 1.7 mu m). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized. Results: The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated. Conclusion: The proposed method was utilized for monitoring of olopatadine in human tear.
PB - Future Sci Ltd, London
T2 - Bioanalysis
T1 - Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method
VL - 9
IS - 24
SP - 1943
EP - 1954
DO - 10.4155/bio-2017-0172
ER -
@article{
author = "Maksić, Jelena and Stajić, Ana and Knežević, Miroslav and Dacić-Krnjaja, Bojana and Jančić-Stojanović, Biljana and Medenica, Mirjana",
year = "2017",
abstract = "Aim: The objective of the study was development of hydrophilic interaction liquid chromatography-ESI/MS/MS method for the determination of olopatadine in tear matrix. Materials & methods: Separation was performed on Acquity BEH amide column (2.1 x 100 mm, 1.7 mu m). The mobile phase was consisted of 0.1% formic acid in water and acetonitrile. Mianserin hydrochloride was implemented as an internal standard. The artificial tear fluid was used as matrix. The tear samples were collected using Schirmer test strips. For the optimization of ultra pressure liquid chromatography conditions, Box-Benhken design was utilized. Results: The optimal values of the ion source and collision cell parameters were found. Quantification was performed in multiple reaction monitoring mode. The optimized method was fully validated. Conclusion: The proposed method was utilized for monitoring of olopatadine in human tear.",
publisher = "Future Sci Ltd, London",
journal = "Bioanalysis",
title = "Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method",
volume = "9",
number = "24",
pages = "1943-1954",
doi = "10.4155/bio-2017-0172"
}
Maksić, J., Stajić, A., Knežević, M., Dacić-Krnjaja, B., Jančić-Stojanović, B.,& Medenica, M.. (2017). Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method. in Bioanalysis
Future Sci Ltd, London., 9(24), 1943-1954.
https://doi.org/10.4155/bio-2017-0172
Maksić J, Stajić A, Knežević M, Dacić-Krnjaja B, Jančić-Stojanović B, Medenica M. Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method. in Bioanalysis. 2017;9(24):1943-1954.
doi:10.4155/bio-2017-0172 .
Maksić, Jelena, Stajić, Ana, Knežević, Miroslav, Dacić-Krnjaja, Bojana, Jančić-Stojanović, Biljana, Medenica, Mirjana, "Determination of olopatadine in human tears by hydrophilic interaction liquid chromatography-MS/MS method" in Bioanalysis, 9, no. 24 (2017):1943-1954,
https://doi.org/10.4155/bio-2017-0172 . .
