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dc.creatorMajkić-Singh, Nada
dc.creatorBogavac, L
dc.creatorKalimanovska, Vesna
dc.creatorJelić, Z
dc.creatorSpasić, S
dc.date.accessioned2019-09-02T10:46:42Z
dc.date.available2019-09-02T10:46:42Z
dc.date.issued1987
dc.identifier.issn0009-8981
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/28
dc.description.abstractA new method for the determination of xanthine oxidase activity, based on the oxidation of 2, 2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) by use of uricase and peroxidase, is described. The absorbance increase of the oxidized form of ABTS, measured after 10 min at 410 nm is proportional to xanthine oxidase activity. The method is sensitive, precise (CV below 8.3%), and linear up to 20 U/l. The analytical recovery of the ABTS-method was quantitative. Comparison with the UV and colorimetric NBT-method gave good correlation (r ≥ 0.984). Reference values for serum xanthine oxidase activities determined with the new ABTS-method on 83 healthy persons are 0 to 1.20 U/l.en
dc.rightsrestrictedAccess
dc.sourceClinica Chimica Acta
dc.subjectABTSen
dc.subjectEvaluationen
dc.subjectPeroxidaseen
dc.subjectReference valueen
dc.subjectSpectrophotometric assayen
dc.subjectXanthine oxidaseen
dc.titleSpectrophotometric assay of xanthine oxidase with 2,2'-azino-di(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as chromogenen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСпасић, С; Мајкић-Сингх, Нада; Калимановска, Весна; Богавац, Л; Јелић, З;
dc.citation.volume162
dc.citation.issue1
dc.citation.spage29
dc.citation.epage36
dc.citation.other162(1): 29-36
dc.citation.rankM21
dc.identifier.doi10.1016/0009-8981(87)90229-4
dc.identifier.scopus2-s2.0-0023125683
dc.identifier.rcubconv_5725
dc.type.versionpublishedVersion


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