Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants
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2017
Authors
Ušjak, Ljuboš
Petrović, Silvana

Drobac, Milica

Soković, Marina

Stanojković, Tatjana

Ćirić, Ana D.

Niketić, Marjan

Article (Published version)

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Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The... strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils.
Source:
Food & Function, 2017, 8, 1, 278-290Publisher:
- Royal Soc Chemistry, Cambridge
Funding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-173021)
- Characterization and application of fungal metabolites and assessment of new biofungicides potential (RS-173032)
- Biological response modifiers in physiological and pathological conditions (RS-175011)
DOI: 10.1039/c6fo01698g
ISSN: 2042-6496
PubMed: 28009867
WoS: 000395385200024
Scopus: 2-s2.0-85011006734
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PharmacyTY - JOUR AU - Ušjak, Ljuboš AU - Petrović, Silvana AU - Drobac, Milica AU - Soković, Marina AU - Stanojković, Tatjana AU - Ćirić, Ana D. AU - Niketić, Marjan PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2908 AB - Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils. AB - PB - Royal Soc Chemistry, Cambridge T2 - Food & Function T1 - Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants VL - 8 IS - 1 SP - 278 EP - 290 DO - 10.1039/c6fo01698g ER -
@article{ author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan", year = "2017", abstract = "Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils., ", publisher = "Royal Soc Chemistry, Cambridge", journal = "Food & Function", title = "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants", volume = "8", number = "1", pages = "278-290", doi = "10.1039/c6fo01698g" }
Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2017). Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function Royal Soc Chemistry, Cambridge., 8(1), 278-290. https://doi.org/10.1039/c6fo01698g
Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function. 2017;8(1):278-290. doi:10.1039/c6fo01698g .
Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants" in Food & Function, 8, no. 1 (2017):278-290, https://doi.org/10.1039/c6fo01698g . .