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Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity

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2017
2915.pdf (1.592Mb)
Authors
Dinić, Miroslav
Lukić, Jovanka
Đokić, Jelena
Milenković, Marina
Strahinić, Ivana
Golić, Nataša
Begović, Jelena
Article (Published version)
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Abstract
The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activatio...n of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.

Source:
Frontiers in Microbiology, 2017, 8
Publisher:
  • Frontiers Media Sa, Lausanne
Projects:
  • Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan (RS-173019)

DOI: 10.3389/fmicb.2017.00594

ISSN: 1664-302X

PubMed: 28428777

WoS: 000398464300001

Scopus: 2-s2.0-85018364309
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2917
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Pharmacy

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