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dc.creatorDinić, Miroslav
dc.creatorLukić, Jovanka
dc.creatorĐokić, Jelena
dc.creatorMilenković, Marina
dc.creatorStrahinić, Ivana
dc.creatorGolić, Nataša
dc.creatorBegović, Jelena
dc.date.accessioned2019-09-02T12:00:21Z
dc.date.available2019-09-02T12:00:21Z
dc.date.issued2017
dc.identifier.issn1664-302X
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2917
dc.description.abstractThe aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.en
dc.publisherFrontiers Media Sa, Lausanne
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.sourceFrontiers in Microbiology
dc.titleLactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicityen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractЛукић, Јованка; Беговић, Јелена; Голић, Наташа; Страхинић, Ивана; Миленковић, Марина; Ђокић, Јелена; Динић, Мирослав;
dc.citation.volume8
dc.citation.other8: -
dc.citation.rankM21
dc.identifier.wos000398464300001
dc.identifier.doi10.3389/fmicb.2017.00594
dc.identifier.pmid28428777
dc.identifier.scopus2-s2.0-85018364309
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1535/2915.pdf
dc.identifier.rcubconv_3814
dc.type.versionpublishedVersion


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