FarFaR - Pharmacy Repository
University of Belgrade, Faculty of Pharmacy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets

Authorized Users Only
2001
Authors
Zečević, Mira
Živanović, L
Agatonović-Kuštrin, Snežana
Minić, D
Article (Published version)
Metadata
Show full item record
Abstract
A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were gr...eater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.

Source:
Journal of Pharmaceutical and Biomedical Analysis, 2001, 24, 5-6, 1019-1025
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford

DOI: 10.1016/S0731-7085(00)00536-7

ISSN: 0731-7085

PubMed: 11248497

WoS: 000167764200032

Scopus: 2-s2.0-0035096691
[ Google Scholar ]
33
31
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/293
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Zečević, Mira
AU  - Živanović, L
AU  - Agatonović-Kuštrin, Snežana
AU  - Minić, D
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/293
AB  - A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets
VL  - 24
IS  - 5-6
SP  - 1019
EP  - 1025
DO  - 10.1016/S0731-7085(00)00536-7
ER  - 
@article{
author = "Zečević, Mira and Živanović, L and Agatonović-Kuštrin, Snežana and Minić, D",
year = "2001",
abstract = "A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets",
volume = "24",
number = "5-6",
pages = "1019-1025",
doi = "10.1016/S0731-7085(00)00536-7"
}
Zečević, M., Živanović, L., Agatonović-Kuštrin, S.,& Minić, D.. (2001). The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 24(5-6), 1019-1025.
https://doi.org/10.1016/S0731-7085(00)00536-7
Zečević M, Živanović L, Agatonović-Kuštrin S, Minić D. The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis. 2001;24(5-6):1019-1025.
doi:10.1016/S0731-7085(00)00536-7 .
Zečević, Mira, Živanović, L, Agatonović-Kuštrin, Snežana, Minić, D, "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets" in Journal of Pharmaceutical and Biomedical Analysis, 24, no. 5-6 (2001):1019-1025,
https://doi.org/10.1016/S0731-7085(00)00536-7 . .

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB