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dc.creatorZečević, Mira
dc.creatorŽivanović, L
dc.creatorAgatonović-Kuštrin, Snežana
dc.creatorMinić, D
dc.date.accessioned2019-09-02T10:53:25Z
dc.date.available2019-09-02T10:53:25Z
dc.date.issued2001
dc.identifier.issn0731-7085
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/293
dc.description.abstractA multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.titleThe use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tabletsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractAгатоновић-Куштрин, Снежана; Зечевић, Мира; Живановић, Л; Минић, Д;
dc.citation.volume24
dc.citation.issue5-6
dc.citation.spage1019
dc.citation.epage1025
dc.citation.other24(5-6): 1019-1025
dc.citation.rankM22
dc.identifier.wos000167764200032
dc.identifier.doi10.1016/S0731-7085(00)00536-7
dc.identifier.pmid11248497
dc.identifier.scopus2-s2.0-0035096691
dc.identifier.rcubconv_1302
dc.type.versionpublishedVersion


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