Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial
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Michalickova, Danica
Kostić-Vučićević, Marija
Vukašinović-Vesić, Milica
Stojmenović, Tamara
Dikić, Nenad

Anđelković, Marija
Đorđević, Brižita

Tanasković, Bojana P.
Minić, Rajna

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To test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: (V)over dotO(2)max 38.2 +/- 4.9 ml.kg(-1).min(-1), age 23.2 +/- 1.4 years; males: (V)over dotO(2)max 57.5 +/- 9.2 ml.kg(-1).min(-1), age 24.0 +/- 2.4 years, mean +/- SD) were randomized either to the probiotic group (Lactobacillus helveticus Lafti L10, 2 x 10(10) colony-forming units) or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific antibacterial antibody levels of IgM, IgG, and IgA classes were determined for different bacteria in the serum, and in saliva, total and specific antibacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15-20%; mean, 90% confidence interval; p = 0.02) and placebo group (35%, 22-47%; p = 0.02), without observed differences in changes between the groups. No significant changes i...n IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, 22.8 to 35%, p = 0.04) reduction of anti-Enterococcus faecalis IgG was noted in the placebo group, in comparison with the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12-18%, p = 0.04) or in saliva (35%, -1.4 to 53%, p = 0.03). Significantly reduced levels of serum anti-lactic acid bacteria IgA antibodies in the placebo group compared with the probiotic group were detected for Lactobacillus rhamnosus LA68 (24%, 5.8-42%, p = 0.02) and for L. rhamnosus LB64 (15%, 2.7-27%, p = 0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.
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Journal of Strength and Conditioning Research, 2017, 31, 1, 62-70Publisher:
- Lippincott Williams & Wilkins, Philadelphia
Funding / projects:
Note:
- Peer-reviewed manuscript: http://farfar.pharmacy.bg.ac.rs/handle/123456789/3440
DOI: 10.1519/JSC.0000000000001456
ISSN: 1064-8011
PubMed: 27100317
WoS: 000391857900013
Scopus: 2-s2.0-85017484618
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PharmacyTY - JOUR AU - Michalickova, Danica AU - Kostić-Vučićević, Marija AU - Vukašinović-Vesić, Milica AU - Stojmenović, Tamara AU - Dikić, Nenad AU - Anđelković, Marija AU - Đorđević, Brižita AU - Tanasković, Bojana P. AU - Minić, Rajna PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2955 AB - To test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: (V)over dotO(2)max 38.2 +/- 4.9 ml.kg(-1).min(-1), age 23.2 +/- 1.4 years; males: (V)over dotO(2)max 57.5 +/- 9.2 ml.kg(-1).min(-1), age 24.0 +/- 2.4 years, mean +/- SD) were randomized either to the probiotic group (Lactobacillus helveticus Lafti L10, 2 x 10(10) colony-forming units) or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific antibacterial antibody levels of IgM, IgG, and IgA classes were determined for different bacteria in the serum, and in saliva, total and specific antibacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15-20%; mean, 90% confidence interval; p = 0.02) and placebo group (35%, 22-47%; p = 0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, 22.8 to 35%, p = 0.04) reduction of anti-Enterococcus faecalis IgG was noted in the placebo group, in comparison with the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12-18%, p = 0.04) or in saliva (35%, -1.4 to 53%, p = 0.03). Significantly reduced levels of serum anti-lactic acid bacteria IgA antibodies in the placebo group compared with the probiotic group were detected for Lactobacillus rhamnosus LA68 (24%, 5.8-42%, p = 0.02) and for L. rhamnosus LB64 (15%, 2.7-27%, p = 0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses. PB - Lippincott Williams & Wilkins, Philadelphia T2 - Journal of Strength and Conditioning Research T1 - Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial VL - 31 IS - 1 SP - 62 EP - 70 DO - 10.1519/JSC.0000000000001456 ER -
@article{ author = "Michalickova, Danica and Kostić-Vučićević, Marija and Vukašinović-Vesić, Milica and Stojmenović, Tamara and Dikić, Nenad and Anđelković, Marija and Đorđević, Brižita and Tanasković, Bojana P. and Minić, Rajna", year = "2017", abstract = "To test the influence of probiotic supplementation on humoral immune response, a double-blind, placebo-controlled trial was conducted. Thirty athletes (24 males and 6 females, females: (V)over dotO(2)max 38.2 +/- 4.9 ml.kg(-1).min(-1), age 23.2 +/- 1.4 years; males: (V)over dotO(2)max 57.5 +/- 9.2 ml.kg(-1).min(-1), age 24.0 +/- 2.4 years, mean +/- SD) were randomized either to the probiotic group (Lactobacillus helveticus Lafti L10, 2 x 10(10) colony-forming units) or to the placebo group. Serum and saliva samples were collected at the baseline and after 14 weeks. Total and specific antibacterial antibody levels of IgM, IgG, and IgA classes were determined for different bacteria in the serum, and in saliva, total and specific antibacterial IgA levels were examined. Total IgM was elevated in both probiotic (18%, 15-20%; mean, 90% confidence interval; p = 0.02) and placebo group (35%, 22-47%; p = 0.02), without observed differences in changes between the groups. No significant changes in IgM levels specific for tested bacteria were found. Total IgG level was constant in both groups. A significant (16%, 22.8 to 35%, p = 0.04) reduction of anti-Enterococcus faecalis IgG was noted in the placebo group, in comparison with the probiotic group. There was a substantial decrease in total IgA level in the placebo group, when measured either in serum (15%, 12-18%, p = 0.04) or in saliva (35%, -1.4 to 53%, p = 0.03). Significantly reduced levels of serum anti-lactic acid bacteria IgA antibodies in the placebo group compared with the probiotic group were detected for Lactobacillus rhamnosus LA68 (24%, 5.8-42%, p = 0.02) and for L. rhamnosus LB64 (15%, 2.7-27%, p = 0.02). Probiotic administration could have beneficial effects on systemic humoral and mucosal immune responses.", publisher = "Lippincott Williams & Wilkins, Philadelphia", journal = "Journal of Strength and Conditioning Research", title = "Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial", volume = "31", number = "1", pages = "62-70", doi = "10.1519/JSC.0000000000001456" }
Michalickova, D., Kostić-Vučićević, M., Vukašinović-Vesić, M., Stojmenović, T., Dikić, N., Anđelković, M., Đorđević, B., Tanasković, B. P.,& Minić, R.. (2017). Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial. in Journal of Strength and Conditioning Research Lippincott Williams & Wilkins, Philadelphia., 31(1), 62-70. https://doi.org/10.1519/JSC.0000000000001456
Michalickova D, Kostić-Vučićević M, Vukašinović-Vesić M, Stojmenović T, Dikić N, Anđelković M, Đorđević B, Tanasković BP, Minić R. Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial. in Journal of Strength and Conditioning Research. 2017;31(1):62-70. doi:10.1519/JSC.0000000000001456 .
Michalickova, Danica, Kostić-Vučićević, Marija, Vukašinović-Vesić, Milica, Stojmenović, Tamara, Dikić, Nenad, Anđelković, Marija, Đorđević, Brižita, Tanasković, Bojana P., Minić, Rajna, "Lactobacillus helveticus lafti L10 supplementation modulates mucosal and humoral immunity in elite athletes: A randomized, double-blind, placebo-controlled trial" in Journal of Strength and Conditioning Research, 31, no. 1 (2017):62-70, https://doi.org/10.1519/JSC.0000000000001456 . .