Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study
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2017
Authors
Renieri, Elisavet A.Sfakianakis, Dimitris G.
Alegakis, Athanasios A.
Safenkova, Irina V.

Buha, Aleksandra

Matović, Vesna

Tzardi, Maria
Dzantiev, Boris B.
Divanach, Pascal
Kentouri, Maroudio
Tsatsakis, Aristidis

Article (Published version)

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Cadmium (Cd) has proved to be associated with numerous toxic effects in aquatic organisms via waterbome exposure. With a view to investigate Cd toxicity along a broad spectrum of exposures reaching from environmental to toxic, we employed adult zebrafish (Danio rerio) for an in vivo study. A number of 10 fish per tank were placed in 40 L tanks and were exposed for 30 days to 0.0, 5.0, 25, 50, 75, 100 and 1000 mu g Cd per liter. There were 2 tanks for each Cd exposure (duplicate experiment). Mortality was recorded daily, dead fish were collected and tissue samples were obtained for histologic observation, whereas remaining tissues were stored for Cd burden determination. Surviving fish were collected at the end of the experiment. Median overall survival (OS) in days was found to be 9.0, 11.0, 8.0 and 7.0 for 25 mu g/L, 50 mu g/L, 75 mu g/L and 100 mu g/L respectively, with all of them showing mortality greater than 50%. Remarkably, fish exposed to the highest Cd concentration (1000 mu g.../L) survived the longest exhibiting a mean OS of 29.2 days. Cd determination in fish tissue was conducted with an in house ICP-MS method and levels ranged from 3.1 to 29.1 ng/mg. Log Cd tissue levels were significantly correlated with the log Cd exposure levels (r = 0.535, p lt 0.001). The highest Cd burden was determined for fish exposed to 1000 mu g Cd /L (mean = 12.2 ng/mg). Histopathology supported these results. Our findings disclose a deviation in toxic responses through the range of Cd concentrations, leading to nonlinear responses. These differentiated responses, could be linked to hormesis phenomena.
Keywords:
Cadmium toxicity / Zebrafish mortality / Nonlinear responseSource:
Environmental Research, 2017, 157, 173-181Publisher:
- Academic Press Inc Elsevier Science, San Diego
DOI: 10.1016/j.envres.2017.05.021
ISSN: 0013-9351
PubMed: 28570961
WoS: 000403524000022
Scopus: 2-s2.0-85019657128
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PharmacyTY - JOUR AU - Renieri, Elisavet A. AU - Sfakianakis, Dimitris G. AU - Alegakis, Athanasios A. AU - Safenkova, Irina V. AU - Buha, Aleksandra AU - Matović, Vesna AU - Tzardi, Maria AU - Dzantiev, Boris B. AU - Divanach, Pascal AU - Kentouri, Maroudio AU - Tsatsakis, Aristidis PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2971 AB - Cadmium (Cd) has proved to be associated with numerous toxic effects in aquatic organisms via waterbome exposure. With a view to investigate Cd toxicity along a broad spectrum of exposures reaching from environmental to toxic, we employed adult zebrafish (Danio rerio) for an in vivo study. A number of 10 fish per tank were placed in 40 L tanks and were exposed for 30 days to 0.0, 5.0, 25, 50, 75, 100 and 1000 mu g Cd per liter. There were 2 tanks for each Cd exposure (duplicate experiment). Mortality was recorded daily, dead fish were collected and tissue samples were obtained for histologic observation, whereas remaining tissues were stored for Cd burden determination. Surviving fish were collected at the end of the experiment. Median overall survival (OS) in days was found to be 9.0, 11.0, 8.0 and 7.0 for 25 mu g/L, 50 mu g/L, 75 mu g/L and 100 mu g/L respectively, with all of them showing mortality greater than 50%. Remarkably, fish exposed to the highest Cd concentration (1000 mu g/L) survived the longest exhibiting a mean OS of 29.2 days. Cd determination in fish tissue was conducted with an in house ICP-MS method and levels ranged from 3.1 to 29.1 ng/mg. Log Cd tissue levels were significantly correlated with the log Cd exposure levels (r = 0.535, p lt 0.001). The highest Cd burden was determined for fish exposed to 1000 mu g Cd /L (mean = 12.2 ng/mg). Histopathology supported these results. Our findings disclose a deviation in toxic responses through the range of Cd concentrations, leading to nonlinear responses. These differentiated responses, could be linked to hormesis phenomena. PB - Academic Press Inc Elsevier Science, San Diego T2 - Environmental Research T1 - Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study VL - 157 SP - 173 EP - 181 DO - 10.1016/j.envres.2017.05.021 ER -
@article{ author = "Renieri, Elisavet A. and Sfakianakis, Dimitris G. and Alegakis, Athanasios A. and Safenkova, Irina V. and Buha, Aleksandra and Matović, Vesna and Tzardi, Maria and Dzantiev, Boris B. and Divanach, Pascal and Kentouri, Maroudio and Tsatsakis, Aristidis", year = "2017", abstract = "Cadmium (Cd) has proved to be associated with numerous toxic effects in aquatic organisms via waterbome exposure. With a view to investigate Cd toxicity along a broad spectrum of exposures reaching from environmental to toxic, we employed adult zebrafish (Danio rerio) for an in vivo study. A number of 10 fish per tank were placed in 40 L tanks and were exposed for 30 days to 0.0, 5.0, 25, 50, 75, 100 and 1000 mu g Cd per liter. There were 2 tanks for each Cd exposure (duplicate experiment). Mortality was recorded daily, dead fish were collected and tissue samples were obtained for histologic observation, whereas remaining tissues were stored for Cd burden determination. Surviving fish were collected at the end of the experiment. Median overall survival (OS) in days was found to be 9.0, 11.0, 8.0 and 7.0 for 25 mu g/L, 50 mu g/L, 75 mu g/L and 100 mu g/L respectively, with all of them showing mortality greater than 50%. Remarkably, fish exposed to the highest Cd concentration (1000 mu g/L) survived the longest exhibiting a mean OS of 29.2 days. Cd determination in fish tissue was conducted with an in house ICP-MS method and levels ranged from 3.1 to 29.1 ng/mg. Log Cd tissue levels were significantly correlated with the log Cd exposure levels (r = 0.535, p lt 0.001). The highest Cd burden was determined for fish exposed to 1000 mu g Cd /L (mean = 12.2 ng/mg). Histopathology supported these results. Our findings disclose a deviation in toxic responses through the range of Cd concentrations, leading to nonlinear responses. These differentiated responses, could be linked to hormesis phenomena.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Environmental Research", title = "Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study", volume = "157", pages = "173-181", doi = "10.1016/j.envres.2017.05.021" }
Renieri, E. A., Sfakianakis, D. G., Alegakis, A. A., Safenkova, I. V., Buha, A., Matović, V., Tzardi, M., Dzantiev, B. B., Divanach, P., Kentouri, M.,& Tsatsakis, A.. (2017). Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study. in Environmental Research Academic Press Inc Elsevier Science, San Diego., 157, 173-181. https://doi.org/10.1016/j.envres.2017.05.021
Renieri EA, Sfakianakis DG, Alegakis AA, Safenkova IV, Buha A, Matović V, Tzardi M, Dzantiev BB, Divanach P, Kentouri M, Tsatsakis A. Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study. in Environmental Research. 2017;157:173-181. doi:10.1016/j.envres.2017.05.021 .
Renieri, Elisavet A., Sfakianakis, Dimitris G., Alegakis, Athanasios A., Safenkova, Irina V., Buha, Aleksandra, Matović, Vesna, Tzardi, Maria, Dzantiev, Boris B., Divanach, Pascal, Kentouri, Maroudio, Tsatsakis, Aristidis, "Nonlinear responses to waterborne cadmium exposure in zebrafish. An in vivo study" in Environmental Research, 157 (2017):173-181, https://doi.org/10.1016/j.envres.2017.05.021 . .