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dc.creatorNovaković, Aleksandra
dc.creatorMarinko, Marija
dc.creatorJanković, Goran
dc.creatorStojanović, Ivan
dc.creatorMilojević, Predrag
dc.creatorNenezić, Dragoslav
dc.creatorKanjuh, Vladimir
dc.creatorYang, Qin
dc.creatorHe, Guo-Wei
dc.date.accessioned2019-09-02T12:01:42Z
dc.date.available2019-09-02T12:01:42Z
dc.date.issued2017
dc.identifier.issn0014-2999
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2972
dc.description.abstractThe aim of the present study was to investigate and characterize vasorelaxant effect of procyanidin B2 on human internal mammary artery (HIMA) as one of the mechanisms of its protective effect against vascular risk. Procyanidin B2 induced strong concentration-dependent relaxation of HIMA rings pre-contracted by phenylephrine. Pretreatment with L-NAME, a NO synthase inhibitor, hydroxocobalamin, a NO scavenger, and ODQ, an inhibitor of soluble guanylate cyclase, significantly inhibited procyanidin B2-induced relaxation of HIMA, while indomethacin, a cyclooxygenase inhibitor, considerably reduced effects of low concentrations. Among K+ channel blockers, iberiotoxin, a selective blocker of large conductance Ca2+-activated K+ channels (BKCa), abolished procyanidin B2-induced relaxation, glibenclamide, a selective ATP-sensitive K+(K-ATP) channels blocker, induced partial inhibition, while 4-aminopyridine, a blocker of voltage-gated K+(K-V) channels, and TRAM-34, an inhibitor of intermediate-conductance Ca2+-activated K+(IKCa) channels, slightly reduced maximal relaxation of HIMA. Further, procyanidin B2 relaxed contraction induced by phenylephrine in Ca2+-free Krebs solution, but had no effect on contraction induced by caffeine. Finally, thapsigargin, a sarcoplasmic reticulum Ca2+-ATPase inhibitor, significantly reduced relaxation of HIMA produced by procyanidin B2. These results demonstrate that procyanidin B2 produces endothelium-dependent relaxation of HIMA pre-contracted by phenylephrine. This effect is primarily the result of an increased NO synthesis and secretion by endothelial cells and partially of prostacyclin, although it involves activation of BKCa and K-ATP, as well as K-V and IKCa channels in high concentrations of procyanidin B2.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175088/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Pharmacology
dc.subjectProcyanidin B2en
dc.subjectHuman internal mammary arteryen
dc.subjectVasorelaxationen
dc.subjectK+ channelsen
dc.subjectIntracellular calciumen
dc.subjectEndotheliumen
dc.titleEndothelium-dependent vasorelaxant effect of procyanidin B2 on human internal mammary arteryen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаринко, Марија; Јанковић, Горан; Стојановић, Иван; Милојевић, Предраг; Ненезић, Драгослав; Кањух, Владимир; Хе, Гуо-Wеи; Yанг, Qин; Новаковић, Aлександра;
dc.citation.volume807
dc.citation.spage75
dc.citation.epage81
dc.citation.other807: 75-81
dc.citation.rankM22
dc.identifier.wos000403520300009
dc.identifier.doi10.1016/j.ejphar.2017.04.015
dc.identifier.pmid28414054
dc.identifier.scopus2-s2.0-85017480494
dc.identifier.rcubconv_3875
dc.type.versionpublishedVersion


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