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dc.creatorMalesević, Milka
dc.creatorVasiljević, Zorica
dc.creatorSovtić, Aleksandar
dc.creatorFilipić, Brankica
dc.creatorNovović, Katarina
dc.creatorKojić, Milan
dc.creatorJovčić, Branko
dc.date.accessioned2019-09-02T12:01:46Z
dc.date.available2019-09-02T12:01:46Z
dc.date.issued2017
dc.identifier.issn2047-2994
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2974
dc.description.abstractBackground: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). Methods: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed. Results: Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene. Conclusion: Variations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.en
dc.publisherBiomed Central Ltd, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.sourceAntimicrobial Resistance and Infection Control
dc.subjectBurkholderia cenocepacia complexen
dc.subjectCystic fibrosisen
dc.subjectEpidemic strainen
dc.subjectVirulenceen
dc.titleVirulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patientsen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractНововић, Катарина; Совтић, Aлександар; Малесевић, Милка; Јовчић, Бранко; Којић, Милан; Филипић, Бранкица; Васиљевић, Зорица;
dc.citation.volume6
dc.citation.other6: -
dc.citation.rankM21
dc.identifier.wos000403478100002
dc.identifier.doi10.1186/s13756-017-0215-y
dc.identifier.pmid28593045
dc.identifier.scopus2-s2.0-85020227696
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1584/2972.pdf
dc.identifier.rcubconv_3873
dc.type.versionpublishedVersion


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