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The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives

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Authors
Agbaba, Danica
Milojević, Z
Erić, Slavica
Aleksić, Mirjana
Marković, G
Solujić, M
Article (Published version)
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Abstract
A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a sign...ificant reduction of the dexamethasone sodium phosphate content of nasal drops.

Keywords:
Benzalkonium chloride / Dexamethasone / HPTLC / Methyl para-hydroxybenzoate / Xylometazoline
Source:
Journal of Planar Chromatography - Modern TLC, 2001, 14, 5, 322-325
Publisher:
  • Research Inst Medicinal Plants, Budakalasz

ISSN: 0933-4173

WoS: 000178518300003

Scopus: 2-s2.0-0035216901
[ Google Scholar ]
12
11
Handle
https://hdl.handle.net/21.15107/rcub_farfar_298
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/298
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Agbaba, Danica
AU  - Milojević, Z
AU  - Erić, Slavica
AU  - Aleksić, Mirjana
AU  - Marković, G
AU  - Solujić, M
PY  - 2001
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/298
AB  - A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a significant reduction of the dexamethasone sodium phosphate content of nasal drops.
PB  - Research Inst Medicinal Plants, Budakalasz
T2  - Journal of Planar Chromatography - Modern TLC
T1  - The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives
VL  - 14
IS  - 5
SP  - 322
EP  - 325
UR  - https://hdl.handle.net/21.15107/rcub_farfar_298
ER  - 
@article{
author = "Agbaba, Danica and Milojević, Z and Erić, Slavica and Aleksić, Mirjana and Marković, G and Solujić, M",
year = "2001",
abstract = "A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a significant reduction of the dexamethasone sodium phosphate content of nasal drops.",
publisher = "Research Inst Medicinal Plants, Budakalasz",
journal = "Journal of Planar Chromatography - Modern TLC",
title = "The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives",
volume = "14",
number = "5",
pages = "322-325",
url = "https://hdl.handle.net/21.15107/rcub_farfar_298"
}
Agbaba, D., Milojević, Z., Erić, S., Aleksić, M., Marković, G.,& Solujić, M.. (2001). The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives. in Journal of Planar Chromatography - Modern TLC
Research Inst Medicinal Plants, Budakalasz., 14(5), 322-325.
https://hdl.handle.net/21.15107/rcub_farfar_298
Agbaba D, Milojević Z, Erić S, Aleksić M, Marković G, Solujić M. The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives. in Journal of Planar Chromatography - Modern TLC. 2001;14(5):322-325.
https://hdl.handle.net/21.15107/rcub_farfar_298 .
Agbaba, Danica, Milojević, Z, Erić, Slavica, Aleksić, Mirjana, Marković, G, Solujić, M, "The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives" in Journal of Planar Chromatography - Modern TLC, 14, no. 5 (2001):322-325,
https://hdl.handle.net/21.15107/rcub_farfar_298 .

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