The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives
Abstract
A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a sign...ificant reduction of the dexamethasone sodium phosphate content of nasal drops.
Keywords:
Benzalkonium chloride / Dexamethasone / HPTLC / Methyl para-hydroxybenzoate / XylometazolineSource:
Journal of Planar Chromatography - Modern TLC, 2001, 14, 5, 322-325Publisher:
- Springer Nature
DOI: 10.1556/JPC.14.2001.5.3
ISSN: 0933-4173
WoS: 000178518300003
Scopus: 2-s2.0-0035216901
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Institution/Community
PharmacyTY - JOUR AU - Agbaba, Danica AU - Milojević, Z AU - Erić, Slavica AU - Aleksić, Mirjana AU - Marković, G AU - Solujić, M PY - 2001 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/298 AB - A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a significant reduction of the dexamethasone sodium phosphate content of nasal drops. PB - Springer Nature T2 - Journal of Planar Chromatography - Modern TLC T1 - The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives VL - 14 IS - 5 SP - 322 EP - 325 DO - 10.1556/JPC.14.2001.5.3 UR - https://hdl.handle.net/21.15107/rcub_farfar_298 ER -
@article{ author = "Agbaba, Danica and Milojević, Z and Erić, Slavica and Aleksić, Mirjana and Marković, G and Solujić, M", year = "2001", abstract = "A simple and reliable HPTLC method has been developed and validated for the determination of dexamethasone (as the sodium phosphate salt) and xylometazoline (as the chloride). Assay of the compounds was performed on silica gel HPTLC plates with spherical particles by development with the mobile phase ethyl acetate-acetonitrile-diethylamine-water, 3 + 3 + 1 + 1 (v/v). A TLC scanner set at lambda = 240 nm was used for direct evaluation of chromatograms in reflectance/absorbance mode. The validation data linearity (R > 0.996), precision (RSD = 1.67-2.77%), accuracy (recovery = 95.95-100.69%) and LOD and LOQ (ng levels) were determined and found to be satisfactory. Assay of dexamethasone and xylometazoline in nasal drops was performed simultaneously with assay of preservatives such as methyl para-hydroxybenzoate. The effect of the most commonly used preservative, benzalkonium chloride on the concentrations of active substances was investigated; it was found that this compound led to a significant reduction of the dexamethasone sodium phosphate content of nasal drops.", publisher = "Springer Nature", journal = "Journal of Planar Chromatography - Modern TLC", title = "The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives", volume = "14", number = "5", pages = "322-325", doi = "10.1556/JPC.14.2001.5.3", url = "https://hdl.handle.net/21.15107/rcub_farfar_298" }
Agbaba, D., Milojević, Z., Erić, S., Aleksić, M., Marković, G.,& Solujić, M.. (2001). The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives. in Journal of Planar Chromatography - Modern TLC Springer Nature., 14(5), 322-325. https://doi.org/10.1556/JPC.14.2001.5.3 https://hdl.handle.net/21.15107/rcub_farfar_298
Agbaba D, Milojević Z, Erić S, Aleksić M, Marković G, Solujić M. The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives. in Journal of Planar Chromatography - Modern TLC. 2001;14(5):322-325. doi:10.1556/JPC.14.2001.5.3 https://hdl.handle.net/21.15107/rcub_farfar_298 .
Agbaba, Danica, Milojević, Z, Erić, Slavica, Aleksić, Mirjana, Marković, G, Solujić, M, "The high-performance thin-layer chromatographic determination of dexamethasone and xylometazoline in nasal drops containing different preservatives" in Journal of Planar Chromatography - Modern TLC, 14, no. 5 (2001):322-325, https://doi.org/10.1556/JPC.14.2001.5.3 ., https://hdl.handle.net/21.15107/rcub_farfar_298 .