Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
2017
Аутори
Filipić, BrankicaMalesević, Milka
Vasiljević, Zorica
Lukić, Jovanka
Novović, Katarina
Kojić, Milan
Jovčić, Branko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one amon...g patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.
Извор:
Frontiers in Cellular and Infection Microbiology, 2017, 7Издавач:
- Frontiers Media Sa, Lausanne
Финансирање / пројекти:
- Изучавање гена и молекуларних механизама у основи пробиотичке активности бактерија млечне киселине изолованих са подручја западног Балкана (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.3389/fcimb.2017.00224
ISSN: 2235-2988
PubMed: 28611955
WoS: 000402107300001
Scopus: 2-s2.0-85027517077
Институција/група
PharmacyTY - JOUR AU - Filipić, Brankica AU - Malesević, Milka AU - Vasiljević, Zorica AU - Lukić, Jovanka AU - Novović, Katarina AU - Kojić, Milan AU - Jovčić, Branko PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2999 AB - Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Cellular and Infection Microbiology T1 - Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin VL - 7 DO - 10.3389/fcimb.2017.00224 ER -
@article{ author = "Filipić, Brankica and Malesević, Milka and Vasiljević, Zorica and Lukić, Jovanka and Novović, Katarina and Kojić, Milan and Jovčić, Branko", year = "2017", abstract = "Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Cellular and Infection Microbiology", title = "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin", volume = "7", doi = "10.3389/fcimb.2017.00224" }
Filipić, B., Malesević, M., Vasiljević, Z., Lukić, J., Novović, K., Kojić, M.,& Jovčić, B.. (2017). Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology Frontiers Media Sa, Lausanne., 7. https://doi.org/10.3389/fcimb.2017.00224
Filipić B, Malesević M, Vasiljević Z, Lukić J, Novović K, Kojić M, Jovčić B. Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology. 2017;7. doi:10.3389/fcimb.2017.00224 .
Filipić, Brankica, Malesević, Milka, Vasiljević, Zorica, Lukić, Jovanka, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin" in Frontiers in Cellular and Infection Microbiology, 7 (2017), https://doi.org/10.3389/fcimb.2017.00224 . .