Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach
2017
Autori
Joksić, GordanaMicić, Mileva
Filipović, Jelena
Drakulić, Dunja
Stanojlović, Miloš
Čalija, Bojan
Valenta Sobot, Ana
Demajo, Miroslav
Nilsson, Robert
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal... for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
Izvor:
Acta veterinaria, 2017, 67, 1, 1-10Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Radiosenzitivnost humanog genoma (RS-MESTD-Basic Research (BR or ON)-173046)
DOI: 10.1515/acve-2017-0001
ISSN: 0567-8315
WoS: 000401672900001
Scopus: 2-s2.0-85016770699
Institucija/grupa
PharmacyTY - JOUR AU - Joksić, Gordana AU - Micić, Mileva AU - Filipović, Jelena AU - Drakulić, Dunja AU - Stanojlović, Miloš AU - Čalija, Bojan AU - Valenta Sobot, Ana AU - Demajo, Miroslav AU - Nilsson, Robert PY - 2017 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3015 AB - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta veterinaria T1 - Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach VL - 67 IS - 1 SP - 1 EP - 10 DO - 10.1515/acve-2017-0001 ER -
@article{ author = "Joksić, Gordana and Micić, Mileva and Filipović, Jelena and Drakulić, Dunja and Stanojlović, Miloš and Čalija, Bojan and Valenta Sobot, Ana and Demajo, Miroslav and Nilsson, Robert", year = "2017", abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta veterinaria", title = "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach", volume = "67", number = "1", pages = "1-10", doi = "10.1515/acve-2017-0001" }
Joksić, G., Micić, M., Filipović, J., Drakulić, D., Stanojlović, M., Čalija, B., Valenta Sobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 1-10. https://doi.org/10.1515/acve-2017-0001
Joksić G, Micić M, Filipović J, Drakulić D, Stanojlović M, Čalija B, Valenta Sobot A, Demajo M, Nilsson R. Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria. 2017;67(1):1-10. doi:10.1515/acve-2017-0001 .
Joksić, Gordana, Micić, Mileva, Filipović, Jelena, Drakulić, Dunja, Stanojlović, Miloš, Čalija, Bojan, Valenta Sobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach" in Acta veterinaria, 67, no. 1 (2017):1-10, https://doi.org/10.1515/acve-2017-0001 . .