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Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach
dc.creator | Joksić, Gordana | |
dc.creator | Micić, Mileva | |
dc.creator | Filipović, Jelena | |
dc.creator | Drakulić, Dunja | |
dc.creator | Stanojlović, Miloš | |
dc.creator | Čalija, Bojan | |
dc.creator | Valenta Sobot, Ana | |
dc.creator | Demajo, Miroslav | |
dc.creator | Nilsson, Robert | |
dc.date.accessioned | 2019-09-02T12:02:42Z | |
dc.date.available | 2019-09-02T12:02:42Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3015 | |
dc.description.abstract | The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals. | en |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173046/RS// | |
dc.rights | openAccess | |
dc.source | Acta veterinaria | |
dc.title | Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Филиповић, Јелена; Чалија, Бојан; Мицић, Милева; Нилссон, Роберт; Дракулић, Дуња; Станојловић, Милош; Валента Собот, Aна; Демајо, Мирослав; Јоксић, Гордана; | |
dc.citation.volume | 67 | |
dc.citation.issue | 1 | |
dc.citation.spage | 1 | |
dc.citation.epage | 10 | |
dc.citation.other | 67(1): 1-10 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000401672900001 | |
dc.identifier.doi | 10.1515/acve-2017-0001 | |
dc.identifier.scopus | 2-s2.0-85016770699 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs//bitstream/id/1623/3013.pdf | |
dc.type.version | publishedVersion |