pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).
TY - JOUR
AU - Savić, Jelena
AU - Marković, Bojan
AU - Vitnik, Vesna
AU - Dilber, Sanda
PY - 2018
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/3032
AB - pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).
PB - Univerzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac
T2 - Kragujevac Journal of Science
T1 - Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography
IS - 40
SP - 103
EP - 111
DO - 10.5937/KgJSci1840103S
ER -
@article{
author = "Savić, Jelena and Marković, Bojan and Vitnik, Vesna and Dilber, Sanda",
year = "2018",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/3032",
abstract = "pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).",
publisher = "Univerzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac",
journal = "Kragujevac Journal of Science",
title = "Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography",
number = "40",
pages = "103-111",
doi = "10.5937/KgJSci1840103S"
}
Savić J, Marković B, Vitnik V, Dilber S. Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography. Kragujevac Journal of Science. 2018;(40):103-111
Savić, J., Marković, B., Vitnik, V.,& Dilber, S. (2018). Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography.
Kragujevac Journal of ScienceUniverzitet u Kragujevcu - Prirodno-matematički fakultet, Kragujevac.(40), 103-111.
https://doi.org/10.5937/KgJSci1840103S
Savić Jelena, Marković Bojan, Vitnik Vesna, Dilber Sanda, "Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography", no. 40 (2018):103-111,
https://doi.org/10.5937/KgJSci1840103S .
