Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction
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2018
Authors
Cvetanović, AleksandraZengin, Gokhan

Zekovic, Zoran
Svarc-Gajic, Jaroslava
Ražić, Slavica

Damjanović, Ana B.

Magkovic, Pavle
Mitic, Milan
Article (Published version)

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Show full item recordAbstract
Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries ...extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.
Keywords:
Subcritical water extraction / Aronia / HPLC / Antioxidants / Enzyme inhibitors / Cytotoxicity / Antimicrobial activitySource:
Food and Chemical Toxicology, 2018, 121, 458-466Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Pharmacologically active substances and products of medicinal/aromatic plants for pharmaceutical use (RS-31013)
DOI: 10.1016/j.fct.2018.09.045
ISSN: 0278-6915
PubMed: 30248480
WoS: 000449242800051
Scopus: 2-s2.0-85053849364
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Institution/Community
PharmacyTY - JOUR AU - Cvetanović, Aleksandra AU - Zengin, Gokhan AU - Zekovic, Zoran AU - Svarc-Gajic, Jaroslava AU - Ražić, Slavica AU - Damjanović, Ana B. AU - Magkovic, Pavle AU - Mitic, Milan PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3056 AB - Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction VL - 121 SP - 458 EP - 466 DO - 10.1016/j.fct.2018.09.045 ER -
@article{ author = "Cvetanović, Aleksandra and Zengin, Gokhan and Zekovic, Zoran and Svarc-Gajic, Jaroslava and Ražić, Slavica and Damjanović, Ana B. and Magkovic, Pavle and Mitic, Milan", year = "2018", abstract = "Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 degrees C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction", volume = "121", pages = "458-466", doi = "10.1016/j.fct.2018.09.045" }
Cvetanović, A., Zengin, G., Zekovic, Z., Svarc-Gajic, J., Ražić, S., Damjanović, A. B., Magkovic, P.,& Mitic, M.. (2018). Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 121, 458-466. https://doi.org/10.1016/j.fct.2018.09.045
Cvetanović A, Zengin G, Zekovic Z, Svarc-Gajic J, Ražić S, Damjanović AB, Magkovic P, Mitic M. Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction. in Food and Chemical Toxicology. 2018;121:458-466. doi:10.1016/j.fct.2018.09.045 .
Cvetanović, Aleksandra, Zengin, Gokhan, Zekovic, Zoran, Svarc-Gajic, Jaroslava, Ražić, Slavica, Damjanović, Ana B., Magkovic, Pavle, Mitic, Milan, "Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction" in Food and Chemical Toxicology, 121 (2018):458-466, https://doi.org/10.1016/j.fct.2018.09.045 . .