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Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease

Authorized Users Only
2018
Authors
Miljković, Milica
Stefanović, Aleksandra
Vekić, Jelena
Zeljković, Aleksandra
Gojković, Tamara
Simić-Ogrizović, Sanja
Bogavac-Stanojević, Nataša
Cerne, Darko
Ilić, Jasmina
Stefanović, Ivan
Jelić-Ivanović, Zorana
Spasojević-Kalimanovska, Vesna
Kotur-Stevuljević, Jelena
Article (Published version)
Metadata
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Abstract
Introduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p lt 0.01) and arylesterase activity (p lt 0.001) of PON1 as well as its concentration (p lt 0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3... subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p lt 0.05) and ESRD (p lt 0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p lt 0.001) and HDL3 (CKD p lt 0.05; ESRD patients p lt 0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p lt 0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.

Keywords:
Dyslipidaemia / Paraoxonase-1 / HDL subclasses
Source:
Clinical Biochemistry, 2018, 60, 52-58
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)

DOI: 10.1016/j.clinbiochem.2018.08.006

ISSN: 0009-9120

PubMed: 30130521

WoS: 000443999700008

Scopus: 2-s2.0-85052753310
[ Google Scholar ]
21
14
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3067
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Gojković, Tamara
AU  - Simić-Ogrizović, Sanja
AU  - Bogavac-Stanojević, Nataša
AU  - Cerne, Darko
AU  - Ilić, Jasmina
AU  - Stefanović, Ivan
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Kotur-Stevuljević, Jelena
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3067
AB  - Introduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p  lt  0.01) and arylesterase activity (p  lt  0.001) of PON1 as well as its concentration (p  lt  0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3 subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p  lt  0.05) and ESRD (p  lt  0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p  lt  0.001) and HDL3 (CKD p  lt  0.05; ESRD patients p  lt  0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p  lt  0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease
VL  - 60
SP  - 52
EP  - 58
DO  - 10.1016/j.clinbiochem.2018.08.006
ER  - 
@article{
author = "Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Zeljković, Aleksandra and Gojković, Tamara and Simić-Ogrizović, Sanja and Bogavac-Stanojević, Nataša and Cerne, Darko and Ilić, Jasmina and Stefanović, Ivan and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Kotur-Stevuljević, Jelena",
year = "2018",
abstract = "Introduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p  lt  0.01) and arylesterase activity (p  lt  0.001) of PON1 as well as its concentration (p  lt  0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3 subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p  lt  0.05) and ESRD (p  lt  0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p  lt  0.001) and HDL3 (CKD p  lt  0.05; ESRD patients p  lt  0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p  lt  0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease",
volume = "60",
pages = "52-58",
doi = "10.1016/j.clinbiochem.2018.08.006"
}
Miljković, M., Stefanović, A., Vekić, J., Zeljković, A., Gojković, T., Simić-Ogrizović, S., Bogavac-Stanojević, N., Cerne, D., Ilić, J., Stefanović, I., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V.,& Kotur-Stevuljević, J.. (2018). Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 60, 52-58.
https://doi.org/10.1016/j.clinbiochem.2018.08.006
Miljković M, Stefanović A, Vekić J, Zeljković A, Gojković T, Simić-Ogrizović S, Bogavac-Stanojević N, Cerne D, Ilić J, Stefanović I, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Kotur-Stevuljević J. Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease. in Clinical Biochemistry. 2018;60:52-58.
doi:10.1016/j.clinbiochem.2018.08.006 .
Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Zeljković, Aleksandra, Gojković, Tamara, Simić-Ogrizović, Sanja, Bogavac-Stanojević, Nataša, Cerne, Darko, Ilić, Jasmina, Stefanović, Ivan, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Kotur-Stevuljević, Jelena, "Activity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal disease" in Clinical Biochemistry, 60 (2018):52-58,
https://doi.org/10.1016/j.clinbiochem.2018.08.006 . .

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