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dc.creatorMiljković, Milica
dc.creatorStefanović, Aleksandra
dc.creatorVekić, Jelena
dc.creatorZeljković, Aleksandra
dc.creatorGojković, Tamara
dc.creatorSimić-Ogrizović, Sanja
dc.creatorBogavac-Stanojević, Nataša
dc.creatorCerne, Darko
dc.creatorIlić, Jasmina
dc.creatorStefanović, Ivan
dc.creatorJelić-Ivanović, Zorana
dc.creatorSpasojević-Kalimanovska, Vesna
dc.creatorKotur-Stevuljević, Jelena
dc.date.accessioned2019-09-02T12:03:54Z
dc.date.available2019-09-02T12:03:54Z
dc.date.issued2018
dc.identifier.issn0009-9120
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3067
dc.description.abstractIntroduction: Cardiovascular complications, as the main cause of mortality in renal patients, are followed with altered lipoproteins composition. Considering that paraoxonase-1 (PON1) is an anti-oxidative enzyme located mainly on HDL particles, the current study has aim to investigate whether failure of kidney function leads to changes in the distribution of PON1 activity between different HDL subclasses. Materials and methods: In 77 renal patients (21 chronic kidney disease (CKD) and 56 end stage renal disease (ESRD) patients on dialysis) and 20 healthy subjects PON1 activity on HDL2 and HDL3 subclasses was determined by zymogram method that combines gradient gel electrophoresis separation of HDL subclasses and measurement of PON1 activity in the same gel. Results: Serum paraoxonase (p lt 0.01) and arylesterase activity (p lt 0.001) of PON1 as well as its concentration (p lt 0.01) were significantly lower in CKD and ESRD patients compared to controls. Relative proportion of HDL3 subclasses was higher in ESRD patients than in healthy participants, while HDL2 subclasses was significantly decreased in CKD (p lt 0.05) and ESRD (p lt 0.001) patients, as compared to controls. Furthermore, control subjects had higher PON1 activity on HDL2 (CKD and ESRD patients p lt 0.001) and HDL3 (CKD p lt 0.05; ESRD patients p lt 0.001) subclasses in comparison with the both patients groups. Also, significant negative correlation was found between paraoxonase activity of PON1 in serum and creatinine concentration (rho = -0.373, p lt 0.01). Conclusions: This study showed that altered HDL subclasses distribution, changed PON1 activities on different HDL subclasses as well as diminished anti-oxidative protection could be important factors in atherosclerosis development in CKD and ESRD patients.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175035/RS//
dc.rightsrestrictedAccess
dc.sourceClinical Biochemistry
dc.subjectDyslipidaemiaen
dc.subjectParaoxonase-1en
dc.subjectHDL subclassesen
dc.titleActivity of paraoxonase 1 (PON1) on HDL2 and HDL3 subclasses in renal diseaseen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБогавац-Станојевић, Наташа; Котур-Стевуљевић, Јелена; Јелић-Ивановић, Зорана; Спасојевић-Калимановска, Весна; Симић-Огризовић, Сања; Стефановић, Иван; Гојковић, Тамара; Миљковић, Милица; Векић, Јелена; Илић, Јасмина; Церне, Дарко; Стефановић, Aлександра; Зељковић, Aлександра;
dc.citation.volume60
dc.citation.spage52
dc.citation.epage58
dc.citation.other60: 52-58
dc.citation.rankM22
dc.identifier.wos000443999700008
dc.identifier.doi10.1016/j.clinbiochem.2018.08.006
dc.identifier.pmid30130521
dc.identifier.scopus2-s2.0-85052753310
dc.identifier.rcubconv_4213
dc.type.versionpublishedVersion


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