Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
Samo za registrovane korisnike
2018
Autori
Živković, LadaBajić, Vladan
Dekanski, Dragana
Pirković, Andrea
Giampieri, Francesca
Gasparrini, Massimiliano
Mazzoni, Luca
Potparević, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p lt 0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from ox...idative damage induced by hydrogen peroxide in vitro.
Izvor:
Food and Chemical Toxicology, 2018, 119, 61-65Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.1016/j.fct.2018.05.034
ISSN: 0278-6915
PubMed: 29763681
WoS: 000443664200009
Scopus: 2-s2.0-85048158869
Institucija/grupa
PharmacyTY - JOUR AU - Živković, Lada AU - Bajić, Vladan AU - Dekanski, Dragana AU - Pirković, Andrea AU - Giampieri, Francesca AU - Gasparrini, Massimiliano AU - Mazzoni, Luca AU - Potparević, Biljana PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3092 AB - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p lt 0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro VL - 119 SP - 61 EP - 65 DO - 10.1016/j.fct.2018.05.034 ER -
@article{ author = "Živković, Lada and Bajić, Vladan and Dekanski, Dragana and Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Potparević, Biljana", year = "2018", abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p lt 0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro", volume = "119", pages = "61-65", doi = "10.1016/j.fct.2018.05.034" }
Živković, L., Bajić, V., Dekanski, D., Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 119, 61-65. https://doi.org/10.1016/j.fct.2018.05.034
Živković L, Bajić V, Dekanski D, Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65. doi:10.1016/j.fct.2018.05.034 .
Živković, Lada, Bajić, Vladan, Dekanski, Dragana, Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65, https://doi.org/10.1016/j.fct.2018.05.034 . .