Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
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2018
Authors
Stevanović, MilenaĐosić, Marija
Janković, Ana

Kojić, Vesna

Vukašinović-Sekulić, Maja
Stojanović, Jovica
Odović, Jadranka

Crevar-Sakač, Milkica

Rhee, Kyong Yop
Mišković-Stanković, Vesna

Article (Published version)

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Show full item recordAbstract
Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed a...gainst Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
Keywords:
biocomposite / coatings / electrophoretic deposition / surface analysis / antibacterial activitySource:
ACS Biomaterials Science & Engineering, 2018, 4, 12, 3994-4007Publisher:
- Amer Chemical Soc, Washington
Projects:
- Basic Science Research Program of the Ministry of Education, Science and Technology of Korea - 2018R1A2B5A02023190
DOI: 10.1021/acsbiomaterials.8b00859
ISSN: 2373-9878