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Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study

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2018
3134.pdf (833.3Kb)
Authors
Jovanović, Dragana
Roksandić-Milenković, Marina
Kotur-Stevuljević, Jelena
Marković, Jelena
Ceriman, Vesna
Kontić, Milica
Skodrić-Trifunović, Vesna
Article (Published version)
Metadata
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Abstract
Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue sa...mples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.

Keywords:
Idiopathic pulmonary fibrosis (IPF) / membrane PD-L1 expression / soluble PD-L1
Source:
Journal of Thoracic Disease, 2018, 10, 12, 6660-6669
Publisher:
  • Ame Publ Co, Shatin
Funding / projects:
  • Clinical and epidemiological study of adverse events in hospital patients (RS-175046)

DOI: 10.21037/jtd.2018.11.16

ISSN: 2072-1439

PubMed: 30746211

WoS: 000456834700070

Scopus: 2-s2.0-85059540040
[ Google Scholar ]
24
20
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3136
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Jovanović, Dragana
AU  - Roksandić-Milenković, Marina
AU  - Kotur-Stevuljević, Jelena
AU  - Marković, Jelena
AU  - Ceriman, Vesna
AU  - Kontić, Milica
AU  - Skodrić-Trifunović, Vesna
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3136
AB  - Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.
PB  - Ame Publ Co, Shatin
T2  - Journal of Thoracic Disease
T1  - Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study
VL  - 10
IS  - 12
SP  - 6660
EP  - 6669
DO  - 10.21037/jtd.2018.11.16
UR  - conv_4304
ER  - 
@article{
author = "Jovanović, Dragana and Roksandić-Milenković, Marina and Kotur-Stevuljević, Jelena and Marković, Jelena and Ceriman, Vesna and Kontić, Milica and Skodrić-Trifunović, Vesna",
year = "2018",
abstract = "Background: Idiopathic pulmonary fibrosis (IPF) has common risk factors with cancer and significant similarities in the pathobiology process, both diseases having poor outcomes. Immune checkpoint PD-L1 has become the target of checkpoint inhibitory therapy that unleashes antitumor T cells and has revolutionized cancer treatment. This is a pilot study exploring membrane immune checkpoint PD-L1 expression in human IPF lung tissue samples and its soluble form, soluble PD-L1 (sPD-L1) plasma concentrations in IPF patients, in order to investigate potential role of PD-L1 as an IPF biomarker. Methods: Twelve human IPF lung tissue samples (formalin-fixed, paraffin-embedded) obtained by surgical biopsy, have been tested for PD-L1 expression by PD-L1 IHC 22C3 pharmDx assay, while plasma samples for examination of sPD-L1 forms, PD-L1 (B7-H1/CD274) blood concentration, originated from 23 patients with IPE who did not undergo surgical biopsy. Results: Membrane PD-L1 expression in IPF lung tissue samples was positive to overexpression of PD-L1 in 9 samples out of 12. Only very few cells in the interstitium have shown a discrete PD-L1 expression, but not of a membrane type. As for sPD-L1 forms, we have found elevated concentrations of sPD-L1 in the serum of IPF patients 314.3 ng/L (117.7-483.1 ng/L), significantly higher compared with healthy control group 91.0 ng/L (52.4-119.7 ng/L), P lt 0.01. Conclusions: For IPF with PD-L1 expression on alveolar macrophages, further studies are necessary to elucidate this phenomenon. Serum sPD-1/PD-L1 is easily detected in clinical practice and should be further evaluated as a potential prognostic or/and predictive biomarker in IPF.",
publisher = "Ame Publ Co, Shatin",
journal = "Journal of Thoracic Disease",
title = "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study",
volume = "10",
number = "12",
pages = "6660-6669",
doi = "10.21037/jtd.2018.11.16",
url = "conv_4304"
}
Jovanović, D., Roksandić-Milenković, M., Kotur-Stevuljević, J., Marković, J., Ceriman, V., Kontić, M.,& Skodrić-Trifunović, V.. (2018). Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease
Ame Publ Co, Shatin., 10(12), 6660-6669.
https://doi.org/10.21037/jtd.2018.11.16
conv_4304
Jovanović D, Roksandić-Milenković M, Kotur-Stevuljević J, Marković J, Ceriman V, Kontić M, Skodrić-Trifunović V. Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study. in Journal of Thoracic Disease. 2018;10(12):6660-6669.
doi:10.21037/jtd.2018.11.16
conv_4304 .
Jovanović, Dragana, Roksandić-Milenković, Marina, Kotur-Stevuljević, Jelena, Marković, Jelena, Ceriman, Vesna, Kontić, Milica, Skodrić-Trifunović, Vesna, "Membrane PD-L1 expression and soluble PD-L1 plasma levels in idiopathic pulmonary fibrosis-a pilot study" in Journal of Thoracic Disease, 10, no. 12 (2018):6660-6669,
https://doi.org/10.21037/jtd.2018.11.16 .,
conv_4304 .

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