Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent

2018
Authors
Cebović, TatjanaArsenijević, Jelena

Drobac, Milica

Živković, Jelena
Šoštarić, Ivan

Maksimović, Zoran

Article (Published version)

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Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antio...xidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.
Keywords:
Thyme / Water extract / Antioxidant / Prooxidant / Cytotoxicity / HPLCSource:
Journal of Food Science and Technology-Mysore, 2018, 55, 2, 560-567Publisher:
- Springer India, New Delhi
Funding / projects:
DOI: 10.1007/s13197-017-2965-1
ISSN: 0022-1155
PubMed: 29391620
WoS: 000423388600013
Scopus: 2-s2.0-85035798705
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PharmacyTY - JOUR AU - Cebović, Tatjana AU - Arsenijević, Jelena AU - Drobac, Milica AU - Živković, Jelena AU - Šoštarić, Ivan AU - Maksimović, Zoran PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3158 AB - Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects. PB - Springer India, New Delhi T2 - Journal of Food Science and Technology-Mysore T1 - Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent VL - 55 IS - 2 SP - 560 EP - 567 DO - 10.1007/s13197-017-2965-1 ER -
@article{ author = "Cebović, Tatjana and Arsenijević, Jelena and Drobac, Milica and Živković, Jelena and Šoštarić, Ivan and Maksimović, Zoran", year = "2018", abstract = "Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.", publisher = "Springer India, New Delhi", journal = "Journal of Food Science and Technology-Mysore", title = "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent", volume = "55", number = "2", pages = "560-567", doi = "10.1007/s13197-017-2965-1" }
Cebović, T., Arsenijević, J., Drobac, M., Živković, J., Šoštarić, I.,& Maksimović, Z.. (2018). Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore Springer India, New Delhi., 55(2), 560-567. https://doi.org/10.1007/s13197-017-2965-1
Cebović T, Arsenijević J, Drobac M, Živković J, Šoštarić I, Maksimović Z. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore. 2018;55(2):560-567. doi:10.1007/s13197-017-2965-1 .
Cebović, Tatjana, Arsenijević, Jelena, Drobac, Milica, Živković, Jelena, Šoštarić, Ivan, Maksimović, Zoran, "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent" in Journal of Food Science and Technology-Mysore, 55, no. 2 (2018):560-567, https://doi.org/10.1007/s13197-017-2965-1 . .