The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified... TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
TY - JOUR
AU - Dekanski, Dragana
AU - Potparević, Biljana
AU - Bajić, Vladan
AU - Živković, Lada
AU - Topalović, Dijana
AU - Sredojević, Dušan
AU - Lazić, Vesna
AU - Nedeljković, Jovan M.
PY - 2018
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3165
AB - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Food and Chemical Toxicology
T1 - Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid
VL - 115
SP - 42
EP - 48
DO - 10.1016/j.fct.2018.02.064
ER -
@article{
author = "Dekanski, Dragana and Potparević, Biljana and Bajić, Vladan and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna and Nedeljković, Jovan M.",
year = "2018",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}
Dekanski, D., Potparević, B., Bajić, V., Živković, L., Topalović, D., Sredojević, D., Lazić, V.,& Nedeljković, J. M.. (2018). Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064
Dekanski D, Potparević B, Bajić V, Živković L, Topalović D, Sredojević D, Lazić V, Nedeljković JM. Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. in Food and Chemical Toxicology. 2018;115:42-48.
doi:10.1016/j.fct.2018.02.064 .
Dekanski, Dragana, Potparević, Biljana, Bajić, Vladan, Živković, Lada, Topalović, Dijana, Sredojević, Dušan, Lazić, Vesna, Nedeljković, Jovan M., "Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid" in Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 . .
