Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material

2018
Authors
Maljurić, Nevena
Golubović, Jelena

Ravnikar, Matjaz
Zigon, Dušan
Štrukelj, Borut
Otašević, Biljana

Article (Published version)
Metadata
Show full item recordAbstract
Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active ...compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.
Source:
Journal of Analytical Methods in Chemistry, 2018Publisher:
- Hindawi Ltd, London
Funding / projects:
DOI: 10.1155/2018/2434691
ISSN: 2090-8865
PubMed: 29675285
WoS: 000426746400001
Scopus: 2-s2.0-85043392753
Collections
Institution/Community
PharmacyTY - JOUR AU - Maljurić, Nevena AU - Golubović, Jelena AU - Ravnikar, Matjaz AU - Zigon, Dušan AU - Štrukelj, Borut AU - Otašević, Biljana PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3182 AB - Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol. PB - Hindawi Ltd, London T2 - Journal of Analytical Methods in Chemistry T1 - Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material DO - 10.1155/2018/2434691 ER -
@article{ author = "Maljurić, Nevena and Golubović, Jelena and Ravnikar, Matjaz and Zigon, Dušan and Štrukelj, Borut and Otašević, Biljana", year = "2018", abstract = "Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.", publisher = "Hindawi Ltd, London", journal = "Journal of Analytical Methods in Chemistry", title = "Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material", doi = "10.1155/2018/2434691" }
Maljurić, N., Golubović, J., Ravnikar, M., Zigon, D., Štrukelj, B.,& Otašević, B.. (2018). Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. in Journal of Analytical Methods in Chemistry Hindawi Ltd, London.. https://doi.org/10.1155/2018/2434691
Maljurić N, Golubović J, Ravnikar M, Zigon D, Štrukelj B, Otašević B. Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. in Journal of Analytical Methods in Chemistry. 2018;. doi:10.1155/2018/2434691 .
Maljurić, Nevena, Golubović, Jelena, Ravnikar, Matjaz, Zigon, Dušan, Štrukelj, Borut, Otašević, Biljana, "Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material" in Journal of Analytical Methods in Chemistry (2018), https://doi.org/10.1155/2018/2434691 . .