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dc.creatorGojković, Tamara
dc.creatorVladimirov, Sandra
dc.creatorSpasojević-Kalimanovska, Vesna
dc.creatorZeljković, Aleksandra
dc.creatorVekić, Jelena
dc.creatorArsenijević, Jelena
dc.creatorĐuričić, Ivana
dc.creatorŠobajić, Slađana
dc.creatorJelić-Ivanović, Zorana
dc.date.accessioned2019-09-02T12:07:04Z
dc.date.available2019-09-02T12:07:04Z
dc.date.issued2018
dc.identifier.issn0009-8981
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3185
dc.description.abstractIntroduction: Cholesterol homeostasis disruption contributes to the development of different pathologies. Non cholesterol sterols (NCSs) serve as cholesterol synthesis markers (desmosterol and lathosterol), and cholesterol absorption surrogate markers (campesterol, stigmasterol and beta-sitosterol). The study aimed to resolve certain new pre-analytical and analytical problems and ensure a reliable and validated method. Materials and methods: Method optimization, validation and stability studies were executed in human serum and plasma. Freeze-thaw cycles were done with and without antioxidant. Gas chromatography-mass spectrometer (GC-MS) was used for NCSs confirmation and plasticizer identification, while GC-flame ionization detector (GC-FID) was used for NCSs quantitation. Results: Infra-and inter-assay variabilities for all NCSs were 2.75-9.55% and 5.80-7.75% for plasma and 3.10-5.72% and 3.05-10.92% for serum, respectively. Recovery studies showed satisfactory percentage errors for all NCSs: 93.4-105.7% in plasma and 87.5-106.9 in serum. Derivatized samples were stable up to 7 days at 20 degrees C and derivatization yield was affected by presence of plasticizers. Fatty acid amids were identified as interfering plastic leachates. Statistically different NCSs concentrations were observed after the 1st freeze-thaw cycle, in antioxidant-free samples, and after the 4th cycle in antioxidant-enriched samples. Conclusions: All of the in-house procedures proved to be useful for minimizing the preanalytical and analytical variations, as proven by the validation results.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175035/RS//
dc.rightsrestrictedAccess
dc.sourceClinica Chimica Acta
dc.subjectMethod validationen
dc.subjectSterolsen
dc.subjectStabilityen
dc.subjectFreeze-thawen
dc.subjectPlasticizersen
dc.titlePreanalytical and analytical challenges in gas chromatographic determination of cholesterol synthesis and absorption markersen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВекић, Јелена; Спасојевић-Калимановска, Весна; Гојковић, Тамара; Ђуричић, Ивана; Јелић-Ивановић, Зорана; Шобајић, Слађана; Aрсенијевић, Јелена; Зељковић, Aлександра; Владимиров, Сандра;
dc.citation.volume478
dc.citation.spage74
dc.citation.epage81
dc.citation.other478: 74-81
dc.citation.rankM21
dc.identifier.wos000425070000013
dc.identifier.doi10.1016/j.cca.2017.12.032
dc.identifier.pmid29274328
dc.identifier.scopus2-s2.0-85039173890
dc.identifier.rcubconv_4050
dc.type.versionpublishedVersion


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