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dc.creatorMarinko, Marija
dc.creatorJanković, Goran
dc.creatorNenezić, Dragoslav
dc.creatorMilojević, Predrag
dc.creatorStojanović, Ivan
dc.creatorKanjuh, Vladimir
dc.creatorNovaković, Aleksandra
dc.date.accessioned2019-09-02T12:07:12Z
dc.date.available2019-09-02T12:07:12Z
dc.date.issued2018
dc.identifier.issn0951-418X
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3191
dc.description.abstractIn this study, we aimed to investigate relaxant effect of flavanol (-)-epicatechin on the isolated human saphenous vein (HSV), as a part of its cardioprotective action, and to define the mechanisms underlying this vasorelaxation. (-)-Epicatechin induced a concentration-dependent relaxation of HSV pre-contracted by phenylephrine. Among K+ channel blockers, 4-aminopyridine, margatoxin, and iberiotoxin significantly inhibited relaxation of HSV, while glibenclamide considerably reduced effects of the high concentrations of (-)-epicatechin. Additionally, (-)-epicatechin relaxed contraction induced by 80 mM K+, whereas in the presence of nifedipine produced partial relaxation of HSV rings pre-contracted by phenylephrine. In Ca2+-free solution, (-)-epicatechin relaxed contraction induced by phenylephrine, but had no effect on contraction induced by caffeine. A sarcoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin, significantly reduced relaxation of HSV produced by (-)-epicatechin. These results demonstrate that (-)-epicatechin produces endothelium-independent relaxation of isolated HSV rings. Vasorelaxation to (-)-epicatechin probably involves activation of 4-aminopyridine- and margatoxin-sensitive K-V channels, BKCa channels, and at least partly, K-ATP channels. In addition, not only the inhibition of extracellular Ca2+ influx, but regulation of the intracellular Ca2+ release, via inositol-trisphosphate receptors and reuptake into sarcoplasmic reticulum, via stimulation of Ca2+-ATPase, as well, most likely participate in (-)-epicatechin-induced relaxation of HSV.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175088/RS//
dc.rightsrestrictedAccess
dc.sourcePhytotherapy Research
dc.subjectepicatechinen
dc.subjectextracellular calciumen
dc.subjecthuman saphenous veinen
dc.subjectintracellular calciumen
dc.subjectK+ channelsen
dc.subjectvasorelaxationen
dc.title(-)-Epicatechin-induced relaxation of isolated human saphenous vein: Roles of K+ and Ca2+ channelsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈанковић, Горан; Маринко, Марија; Ненезић, Драгослав; Новаковић, Aлександра; Милојевић, Предраг; Стојановић, Иван; Кањух, Владимир;
dc.citation.volume32
dc.citation.issue2
dc.citation.spage267
dc.citation.epage275
dc.citation.other32(2): 267-275
dc.citation.rankM21
dc.identifier.wos000424287300008
dc.identifier.doi10.1002/ptr.5969
dc.identifier.pmid29193528
dc.identifier.scopus2-s2.0-85036571538
dc.identifier.rcubconv_4044
dc.type.versionpublishedVersion


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