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(-)-Epicatechin-induced relaxation of isolated human saphenous vein: Roles of K+ and Ca2+ channels
dc.creator | Marinko, Marija | |
dc.creator | Janković, Goran | |
dc.creator | Nenezić, Dragoslav | |
dc.creator | Milojević, Predrag | |
dc.creator | Stojanović, Ivan | |
dc.creator | Kanjuh, Vladimir | |
dc.creator | Novaković, Aleksandra | |
dc.date.accessioned | 2019-09-02T12:07:12Z | |
dc.date.available | 2019-09-02T12:07:12Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0951-418X | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3191 | |
dc.description.abstract | In this study, we aimed to investigate relaxant effect of flavanol (-)-epicatechin on the isolated human saphenous vein (HSV), as a part of its cardioprotective action, and to define the mechanisms underlying this vasorelaxation. (-)-Epicatechin induced a concentration-dependent relaxation of HSV pre-contracted by phenylephrine. Among K+ channel blockers, 4-aminopyridine, margatoxin, and iberiotoxin significantly inhibited relaxation of HSV, while glibenclamide considerably reduced effects of the high concentrations of (-)-epicatechin. Additionally, (-)-epicatechin relaxed contraction induced by 80 mM K+, whereas in the presence of nifedipine produced partial relaxation of HSV rings pre-contracted by phenylephrine. In Ca2+-free solution, (-)-epicatechin relaxed contraction induced by phenylephrine, but had no effect on contraction induced by caffeine. A sarcoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin, significantly reduced relaxation of HSV produced by (-)-epicatechin. These results demonstrate that (-)-epicatechin produces endothelium-independent relaxation of isolated HSV rings. Vasorelaxation to (-)-epicatechin probably involves activation of 4-aminopyridine- and margatoxin-sensitive K-V channels, BKCa channels, and at least partly, K-ATP channels. In addition, not only the inhibition of extracellular Ca2+ influx, but regulation of the intracellular Ca2+ release, via inositol-trisphosphate receptors and reuptake into sarcoplasmic reticulum, via stimulation of Ca2+-ATPase, as well, most likely participate in (-)-epicatechin-induced relaxation of HSV. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175088/RS// | |
dc.rights | restrictedAccess | |
dc.source | Phytotherapy Research | |
dc.subject | epicatechin | en |
dc.subject | extracellular calcium | en |
dc.subject | human saphenous vein | en |
dc.subject | intracellular calcium | en |
dc.subject | K+ channels | en |
dc.subject | vasorelaxation | en |
dc.title | (-)-Epicatechin-induced relaxation of isolated human saphenous vein: Roles of K+ and Ca2+ channels | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јанковић, Горан; Маринко, Марија; Ненезић, Драгослав; Новаковић, Aлександра; Милојевић, Предраг; Стојановић, Иван; Кањух, Владимир; | |
dc.citation.volume | 32 | |
dc.citation.issue | 2 | |
dc.citation.spage | 267 | |
dc.citation.epage | 275 | |
dc.citation.other | 32(2): 267-275 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000424287300008 | |
dc.identifier.doi | 10.1002/ptr.5969 | |
dc.identifier.pmid | 29193528 | |
dc.identifier.scopus | 2-s2.0-85036571538 | |
dc.type.version | publishedVersion |