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dc.creatorColović, Jelena
dc.creatorRmandić, Milena
dc.creatorMalenović, Anđelija
dc.date.accessioned2019-09-02T12:07:20Z
dc.date.available2019-09-02T12:07:20Z
dc.date.issued2018
dc.identifier.issn1618-2642
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3197
dc.description.abstractNumerous stationary phases have been developed with the aim to provide desired performances during chromatographic analysis of the basic solutes in their protonated form. In this work, the procedure for the characterization of bonded stationary phase performance, when both qualitative and quantitative chromatographic factors were varied in chaotropic chromatography, was proposed. Risperidone and its three impurities were selected as model substances, while acetonitrile content in the mobile phase (20-30%), the pH of the aqueous phase (3.00-5.00), the content of chaotropic agents in the aqueous phase (10-100 mM), type of chaotropic agent (NaClO4, CF3COONa), and stationary phase type (Zorbax Eclipse XDB, Zorbax Extend) were studied as chromatographic factors. The proposed procedure implies the combination of D-optimal experimental design, indirect modeling, and polynomial-modified Gaussian model, while grid point search method was selected for the final choice of the experimental conditions which lead to the best possible stationary phase performance for basic solutes. Good agreement between experimentally obtained chromatogram and simulated chromatogram for chosen experimental conditions (25% acetonitrile, 75 mM of NaClO4, pH 4.00 on Zorbax Eclipse XDB column) confirmed the applicability of the proposed procedure. The additional point was selected for the verification of proposed procedure ability to distinguish changes in solutes' elution order. Simulated chromatogram for 21.5% acetonitrile, 85 mM of NaClO4, pH 5.00 on Zorbax Eclipse XDB column was in line with experimental data. Furthermore, the values of left and right peak half-widths obtained from indirect modeling were used in order to evaluate performances of differently modified stationary phases applying a half-width plots approach. The results from half-width plot approach as well as from the proposed procedure indicate higher efficiency and better separation performance of the stationary phase extra densely bonded and double end-capped with trimethylsilyl group than the stationary phase with the combination of end-capping and bidentate silane bonding for chromatographic analysis of basic solutes in RP-HPLC systems with chaotropic agents.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceAnalytical and Bioanalytical Chemistry
dc.subjectIndirect modelingen
dc.subjectPolynomial modified Gaussian modelen
dc.subjectChromatogram simulationen
dc.subjectHalf-width plots approachen
dc.titleCharacterization of bonded stationary phase performance as a function of qualitative and quantitative chromatographic factors in chaotropic chromatography with risperidone and its impurities as model substancesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЦоловић, Јелена; Маленовић, Aнђелија; Рмандић, Милена;
dc.citation.volume410
dc.citation.issue20
dc.citation.spage4855
dc.citation.epage4866
dc.citation.other410(20): 4855-4866
dc.citation.rankM21
dc.identifier.wos000438410800009
dc.identifier.doi10.1007/s00216-018-1122-7
dc.identifier.pmid29770836
dc.identifier.scopus2-s2.0-85047116058
dc.identifier.rcubconv_4154
dc.type.versionpublishedVersion


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