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dc.creatorMinić, Rajna
dc.creatorPapić, Zlatko
dc.creatorĐorđević, Brižita
dc.creatorMichalickova, Danica
dc.creatorLlić, Vesna
dc.creatorMathiesen, Geir
dc.creatorŽivković, Irena
dc.creatorPantić, Višnja
dc.creatorDimitrijević, Ljiljana
dc.date.accessioned2019-09-02T12:07:25Z
dc.date.available2019-09-02T12:07:25Z
dc.date.issued2018
dc.identifier.issn1932-6203
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3199
dc.description.abstractThe goal of this work was to elucidate similarities between microorganisms from the perspective of the humoral immune system reactivity in professional athletes. The reactivity of serum IgG of 14 young, individuals was analyzed to 23 selected microorganisms as antigens by use of the in house ELISA. Serum IgM and IgA reactivity was also analyzed and a control group of sex and age matched individuals was used for comparison. The obtained absorbance levels were used as a string of values to correlate the reactivity to different microorganisms. IgM was found to be the most cross reactive antibody class, Pearson's r = 0.7 +/- 0.92, for very distant bacterial species such as Lactobacillus and E. coli. High correlation in IgG levels was found for Gammaproteobacteria and LPS (from E. coli) (r = 0.77 for LPS vs. P. aeruginosa to r = 0.98 for LPS vs. E. coli), whereas this correlation was lower in the control group (r = 0.49 for LPS vs. P. aeruginosa to r = 0.66 for LPS vs. E. coli). The correlation was also analyzed between total IgG and IgG subclasses specific for the same microorganism, and IgG2 was identified as the main subclass recognising different microorganisms, as well as recognising LPS. Upon correlation of IgG with IgA for the same microorganism absence of or negative correlation was found between bacteria-specific IgA and IgG in case of Lactobacillus and Staphylococcusgeni, whereas correlation was absent or positive for Candida albicans, Enterococcusfaecalis, Streptococcus species tested in professional athletes. Opposite results were obtained for the control group. Outlined here is a simple experimental procedure and data analysis which yields functional significance and which can be used for determining the similarities between microorganisms from the aspect of the humoral immune system, for determining the main IgG subclass involved in an immune response as well as for the analysis of different target populations.en
dc.publisherPublic Library Science, San Francisco
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS//
dc.rightsopenAccess
dc.sourcePLoS One
dc.titleProfiling of microorganism-binding serum antibody specificities in professional athletesen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractДимитријевић, Љиљана; Живковић, Ирена; Мицхалицкова, Даница; Минић, Рајна; Пантић, Вишња; Ђорђевић, Брижита; Папић, Златко; Ллић, Весна; Матхиесен, Геир;
dc.citation.volume13
dc.citation.issue9
dc.citation.other13(9): -
dc.citation.rankM22
dc.identifier.wos000445639700011
dc.identifier.doi10.1371/journal.pone.0203665
dc.identifier.pmid30252853
dc.identifier.scopus2-s2.0-85054012311
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1779/3197.pdf
dc.identifier.rcubconv_4157
dc.type.versionpublishedVersion


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