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dc.creatorSljivić, Jasmina
dc.creatorProtić, Ana
dc.creatorMalenović, Anđelija
dc.creatorOtašević, Biljana
dc.creatorZečević, Mira
dc.date.accessioned2019-09-02T12:07:39Z
dc.date.available2019-09-02T12:07:39Z
dc.date.issued2018
dc.identifier.issn0009-5893
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3208
dc.description.abstractIn this paper, an efficient way for robustness testing of gradient elution liquid chromatographic methods is proposed and tested on model mixtures comprising cilazapril, hydrochlorothiazide, and their degradation products, solutes that differ not only in polarities, but also in solubility and absorption characteristics. In general, the robustness could be tested with respect to various responses: resolution, retention factor, selectivity factor, change of detector response, etc. In chromatographic methods, the separation of the adjacent peaks is mandatory, and, consequently, the resolution is usually used as response. In isocratic elution methods, the resolution threshold depends on many factors, such as sizes of adjacent peaks, peak shapes, and asymmetry factor. At the same time, the situation is even more complex in gradient elution methods, because separation depends on a larger number of parameters, such as gradient profile, column geometry, mobile phase flow rate, column equilibration between gradient runs, etc. To ensure baseline separation, the authors propose application of separation criterion (s) as response and indirect modeling in the robustness evaluation. Examined response in this approach is represented by the difference between the retention time of the beginning of the peak and the retention time of the end of the previously eluting peak of the critical pair. Moreover, the proposed methodology included reusing experiments from the optimization phase to define a robust chromatographic region without increasing the number of experiments. It was shown that method robustness can be easily and efficiently evaluated by this methodology.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS//
dc.rightsrestrictedAccess
dc.sourceChromatographia
dc.subjectLiquid chromatographyen
dc.subjectRobustness testingen
dc.subjectGradient elutionen
dc.subjectSeparation criterionen
dc.subjectIndirect modelingen
dc.titleSimple and Efficient Solution for Robustness Testing in Gradient Elution Liquid Chromatographic Methodsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Зечевић, Мира; Протић, Aна; Сљивић, Јасмина; Оташевић, Биљана;
dc.citation.volume81
dc.citation.issue8
dc.citation.spage1135
dc.citation.epage1145
dc.citation.other81(8): 1135-1145
dc.citation.rankM23
dc.identifier.wos000439334900002
dc.identifier.doi10.1007/s10337-018-3545-x
dc.identifier.scopus2-s2.0-85047156435
dc.identifier.rcubconv_4170
dc.type.versionpublishedVersion


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