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Mouse strain and sex as determinants of immune response to trivalent influenza vaccine

Authorized Users Only
2018
Authors
Petrović, Raisa
Bufan, Biljana
Arsenović-Ranin, Nevena
Živković, Irena
Minić, Rajna
Radojević, Katarina
Leposavić, Gordana
Article (Published version)
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Abstract
Aims: The study examined the influence of sex and mouse strain on germinal center (GC) reaction and antibody responses to seasonal split trivalent influenza vaccine (TIV). Main methods: C57BL/6 and BALB/c mice of both sexes were immunized with TIV and examined for specific antibody response by ELISA. Splenic T follicular regulatory (Tfr), T follicular helper (Tfh) and GC B cells are detected by flow cytometry. The proliferative response of splenocytes, and concentrations of IFN-gamma and IL-4 upon restimulation with vaccine antigens were examined by 7-AAD staining and ELISA, respectively. Key findings: BALB/c mice developed more robust IgG responses to vaccine type A antigens than their sexmatched C57BL/6 counterparts, while that to B antigen did not differ between strains. In both strains IgG responses against type A vaccine antigens were greater in females than in males. The greater IgG responses correlated with lower splenic Tfr/Tfh and Tfr/GC B cell ratios and greater vaccine antig...en-specific proliferative responses of CD4+ and B cells in splenocyte cultures. In both mouse strains IgG2a(c)/IgG1 ratios were comparable between sexes, but lower in BALB/c than in C57BL/6 mice indicating a shift in Th1/Th2 balance towards Th2 response in BALB/c ones. Consistently, splenocytes from BALB/c mice produced more IL-4 and less IFN-gamma than those from C57BL/6 mice. Significance: The study indicated that magnitude of humoral response to influenza type A haemagglutinins depends on mouse strain and sex, and thereby set background for the vaccination strategies taking into account biological sex, and in a longterm perspective individual differences in immune reactivity.

Keywords:
Mouse genetic background / Influenza vaccine / Antibody response / Germinal center reaction / Sex differences
Source:
Life Sciences, 2018, 207, 117-126
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Projects:
  • Immune system plasticity during aging: Immunomodulatory capacity of oestrogens (RS-175050)

DOI: 10.1016/j.lfs.2018.05.056

ISSN: 0024-3205

PubMed: 29859986

WoS: 000439876800015

Scopus: 2-s2.0-85048462717
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/3216
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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