Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices

2018
Authors
Pavun, Leposava
Uskoković-Marković, Snežana

Jelikić-Stankov, Milena
Đikanović, Daniela
Đurđević, Predrag

Article (Published version)

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We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained... profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.
Keywords:
aluminium / antioxidant activity / quercetin / spectrofluorimetry / spectrophotometrySource:
Czech Journal of Food Sciences, 2018, 36, 3, 233-238Publisher:
- Czech Academy Agricultural Sciences, Prague
Funding / projects:
DOI: 10.17221/211/2017-CJFS
ISSN: 1212-1800
WoS: 000437015500006
Scopus: 2-s2.0-85049382451
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Institution/Community
PharmacyTY - JOUR AU - Pavun, Leposava AU - Uskoković-Marković, Snežana AU - Jelikić-Stankov, Milena AU - Đikanović, Daniela AU - Đurđević, Predrag PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3241 AB - We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers. PB - Czech Academy Agricultural Sciences, Prague T2 - Czech Journal of Food Sciences T1 - Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices VL - 36 IS - 3 SP - 233 EP - 238 DO - 10.17221/211/2017-CJFS ER -
@article{ author = "Pavun, Leposava and Uskoković-Marković, Snežana and Jelikić-Stankov, Milena and Đikanović, Daniela and Đurđević, Predrag", year = "2018", abstract = "We propose a sensitive and selective spectrofluorimetric method for the determination of flavonoids as expressed in 'quercetin equivalent' in apple juices. The method is based on the strong emission of the aluminium(III)-quercetin complex at 480 nm with excitation at 420 nm, and it is successfully applied for the determination of flavonoids in commercial apple juices and compared with results obtained in reference spectrophotometric determination. The flavonoid content in commercial apple juices was found to range from 5.53 to 15.55 mg/l quercetin equivalent. The very good agreement between the two methods indicates the suitability of the proposed spectrofluorimetric method for the precise and accurate determination of flavonoids. In addition, the total polyphenol content was determined spectrophotometrically using the Folin-Ciocalteu (FC) method and the antioxidative activity of the tested juices was tested in a DPPH assay and these values were correlated with each other. The obtained profiles of compounds with antioxidative ability lead us to conclude that fruit juice labels based only on fruit % might sometimes misinform consumers.", publisher = "Czech Academy Agricultural Sciences, Prague", journal = "Czech Journal of Food Sciences", title = "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices", volume = "36", number = "3", pages = "233-238", doi = "10.17221/211/2017-CJFS" }
Pavun, L., Uskoković-Marković, S., Jelikić-Stankov, M., Đikanović, D.,& Đurđević, P.. (2018). Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences Czech Academy Agricultural Sciences, Prague., 36(3), 233-238. https://doi.org/10.17221/211/2017-CJFS
Pavun L, Uskoković-Marković S, Jelikić-Stankov M, Đikanović D, Đurđević P. Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices. in Czech Journal of Food Sciences. 2018;36(3):233-238. doi:10.17221/211/2017-CJFS .
Pavun, Leposava, Uskoković-Marković, Snežana, Jelikić-Stankov, Milena, Đikanović, Daniela, Đurđević, Predrag, "Determination of Flavonoids and Total Polyphenol Contents in Commercial Apple Juices" in Czech Journal of Food Sciences, 36, no. 3 (2018):233-238, https://doi.org/10.17221/211/2017-CJFS . .