Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs

2019
Authors
Milanović, ZVekić, Jelena

Radonjić, V
Ilić-Božović, A
Zeljković, Aleksandra

Janać, Jelena

Spasojević-Kalimanovska, Vesna

Buch, J
Chandrashekar, R
Bojić-Trbojević, Ž
Hajduković, L
Christopher, M.M
Kovačević Filipović, M
Article (Published version)

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Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P lt .001). Dogs with B. canis infection had signif...icantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
Keywords:
acute phase response / apolipoprotein A-1 / high-density lipoprotein / lipoprotein diameter / serum amyloid ASource:
Journal of Veterinary Internal Medicine, 2019, 33, 4, 1686-1694Publisher:
- Blackwell Publishing Inc.
Funding / projects:
- Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-175061)
DOI: 10.1111/jvim.15537
ISSN: 0891-6640
WoS: 000481436400016
Scopus: 2-s2.0-85067417594
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PharmacyTY - JOUR AU - Milanović, Z AU - Vekić, Jelena AU - Radonjić, V AU - Ilić-Božović, A AU - Zeljković, Aleksandra AU - Janać, Jelena AU - Spasojević-Kalimanovska, Vesna AU - Buch, J AU - Chandrashekar, R AU - Bojić-Trbojević, Ž AU - Hajduković, L AU - Christopher, M.M AU - Kovačević Filipović, M PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3253 AB - Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding. PB - Blackwell Publishing Inc. T2 - Journal of Veterinary Internal Medicine T1 - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs VL - 33 IS - 4 SP - 1686 EP - 1694 DO - 10.1111/jvim.15537 ER -
@article{ author = "Milanović, Z and Vekić, Jelena and Radonjić, V and Ilić-Božović, A and Zeljković, Aleksandra and Janać, Jelena and Spasojević-Kalimanovska, Vesna and Buch, J and Chandrashekar, R and Bojić-Trbojević, Ž and Hajduković, L and Christopher, M.M and Kovačević Filipović, M", year = "2019", abstract = "Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.", publisher = "Blackwell Publishing Inc.", journal = "Journal of Veterinary Internal Medicine", title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs", volume = "33", number = "4", pages = "1686-1694", doi = "10.1111/jvim.15537" }
Milanović, Z., Vekić, J., Radonjić, V., Ilić-Božović, A., Zeljković, A., Janać, J., Spasojević-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojić-Trbojević, Ž., Hajduković, L., Christopher, M.M,& Kovačević Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine Blackwell Publishing Inc.., 33(4), 1686-1694. https://doi.org/10.1111/jvim.15537
Milanović Z, Vekić J, Radonjić V, Ilić-Božović A, Zeljković A, Janać J, Spasojević-Kalimanovska V, Buch J, Chandrashekar R, Bojić-Trbojević Ž, Hajduković L, Christopher M, Kovačević Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694. doi:10.1111/jvim.15537 .
Milanović, Z, Vekić, Jelena, Radonjić, V, Ilić-Božović, A, Zeljković, Aleksandra, Janać, Jelena, Spasojević-Kalimanovska, Vesna, Buch, J, Chandrashekar, R, Bojić-Trbojević, Ž, Hajduković, L, Christopher, M.M, Kovačević Filipović, M, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694, https://doi.org/10.1111/jvim.15537 . .