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The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography

Authorized Users Only
2019
Authors
Obradović, Darija
Stavrianidi, A.N
Ustinovich, K.B
Parenago, O.O
Shpigun, O.A
Agbaba, Danica
Article (Published version)
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Abstract
This work presents an investigation of retention characteristics of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction liquid chromatography (NA-HILIC) and supercritical fluid chromatography (SFC). The separation has been carried out by using methanol as a mobile phase modifier with addition of two types of additives (NH 4 HCOO; NH 4 HCOO/HCOOH) and two different stationary phases (diol; mixed-mode diol). The selectivity characteristics were observed based on S-factors, logk-logk plots and radar plots. NA-HILIC vs. SFC retention of tested compounds was also described by considering the molecular properties of the analytes within the LSER analysis. The differences between SFC vs. NA-HILIC retention of imidazoline and serotonin receptor ligands grow with the acid addition to a mobile phase, noticeably on mixed-mode diol stationary phase (S ≥ 87). In addition, the good selectivity performances of the certain NA-HILIC and SFC conditions were confirmed by good... separation of structurally related compounds (α ≥ 2). The molecular basis of NA-HILIC and SFC retention were explained by using Abraham's equation. The dominant analyte descriptors influencing retention were hydrogen bonding and dipolar interactions. The current study will present the theory, and discuss the applicability within the SFC vs. NA-HILIC regimes. In this way, it was provided the placing of two relatively new methods (SFC, NA-HILIC) in the map of modern analytical chromatography in terms of the pharmaceutical analysis.

Keywords:
Linear solvation energy relationship / Non-aqueous hydrophilic interaction liquid chromatography / Retention mechanism / Selectivity / Supercritical fluid chromatography
Source:
Journal of Chromatography A, 2019
Publisher:
  • Elsevier B.V.
Funding / projects:
  • Synthesis, Quantitative Structure and Activity Relationship, Physico-Chemical Characterisation and Analysis of Pharmacologically Active Substances (RS-172033)

DOI: 10.1016/j.chroma.2019.04.054

ISSN: 0021-9673

WoS: 000484871200038

Scopus: 2-s2.0-85065033746
[ Google Scholar ]
3
1
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3282
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Obradović, Darija
AU  - Stavrianidi, A.N
AU  - Ustinovich, K.B
AU  - Parenago, O.O
AU  - Shpigun, O.A
AU  - Agbaba, Danica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3282
AB  - This work presents an investigation of retention characteristics of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction liquid chromatography (NA-HILIC) and supercritical fluid chromatography (SFC). The separation has been carried out by using methanol as a mobile phase modifier with addition of two types of additives (NH 4 HCOO; NH 4 HCOO/HCOOH) and two different stationary phases (diol; mixed-mode diol). The selectivity characteristics were observed based on S-factors, logk-logk plots and radar plots. NA-HILIC vs. SFC retention of tested compounds was also described by considering the molecular properties of the analytes within the LSER analysis. The differences between SFC vs. NA-HILIC retention of imidazoline and serotonin receptor ligands grow with the acid addition to a mobile phase, noticeably on mixed-mode diol stationary phase (S ≥ 87). In addition, the good selectivity performances of the certain NA-HILIC and SFC conditions were confirmed by good separation of structurally related compounds (α ≥ 2). The molecular basis of NA-HILIC and SFC retention were explained by using Abraham's equation. The dominant analyte descriptors influencing retention were hydrogen bonding and dipolar interactions. The current study will present the theory, and discuss the applicability within the SFC vs. NA-HILIC regimes. In this way, it was provided the placing of two relatively new methods (SFC, NA-HILIC) in the map of modern analytical chromatography in terms of the pharmaceutical analysis.
PB  - Elsevier B.V.
T2  - Journal of Chromatography A
T1  - The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography
DO  - 10.1016/j.chroma.2019.04.054
ER  - 
@article{
author = "Obradović, Darija and Stavrianidi, A.N and Ustinovich, K.B and Parenago, O.O and Shpigun, O.A and Agbaba, Danica",
year = "2019",
abstract = "This work presents an investigation of retention characteristics of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction liquid chromatography (NA-HILIC) and supercritical fluid chromatography (SFC). The separation has been carried out by using methanol as a mobile phase modifier with addition of two types of additives (NH 4 HCOO; NH 4 HCOO/HCOOH) and two different stationary phases (diol; mixed-mode diol). The selectivity characteristics were observed based on S-factors, logk-logk plots and radar plots. NA-HILIC vs. SFC retention of tested compounds was also described by considering the molecular properties of the analytes within the LSER analysis. The differences between SFC vs. NA-HILIC retention of imidazoline and serotonin receptor ligands grow with the acid addition to a mobile phase, noticeably on mixed-mode diol stationary phase (S ≥ 87). In addition, the good selectivity performances of the certain NA-HILIC and SFC conditions were confirmed by good separation of structurally related compounds (α ≥ 2). The molecular basis of NA-HILIC and SFC retention were explained by using Abraham's equation. The dominant analyte descriptors influencing retention were hydrogen bonding and dipolar interactions. The current study will present the theory, and discuss the applicability within the SFC vs. NA-HILIC regimes. In this way, it was provided the placing of two relatively new methods (SFC, NA-HILIC) in the map of modern analytical chromatography in terms of the pharmaceutical analysis.",
publisher = "Elsevier B.V.",
journal = "Journal of Chromatography A",
title = "The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography",
doi = "10.1016/j.chroma.2019.04.054"
}
Obradović, D., Stavrianidi, A.N, Ustinovich, K.B, Parenago, O.O, Shpigun, O.A,& Agbaba, D.. (2019). The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography. in Journal of Chromatography A
Elsevier B.V...
https://doi.org/10.1016/j.chroma.2019.04.054
Obradović D, Stavrianidi A, Ustinovich K, Parenago O, Shpigun O, Agbaba D. The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography. in Journal of Chromatography A. 2019;.
doi:10.1016/j.chroma.2019.04.054 .
Obradović, Darija, Stavrianidi, A.N, Ustinovich, K.B, Parenago, O.O, Shpigun, O.A, Agbaba, Danica, "The comparison of retention behaviour of imidazoline and serotonin receptor ligands in non-aqueous hydrophilic interaction chromatography and supercritical fluid chromatography" in Journal of Chromatography A (2019),
https://doi.org/10.1016/j.chroma.2019.04.054 . .

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