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dc.creatorRadulović, Valentina
dc.creatorAleksić, Mara
dc.creatorKapetanović, Vera
dc.creatorRajić, K.K
dc.creatorJovanović, M
dc.creatorMarjanović, I
dc.creatorStojković, M
dc.creatorAgbaba, Danica
dc.date.accessioned2019-09-02T12:09:34Z
dc.date.available2019-09-02T12:09:34Z
dc.date.issued2019
dc.identifier.issn1618-2642
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3286
dc.description.abstractA novel voltammetric method was developed for brimonidine (BRIM) determination in deproteinized aqueous humor, simplifying preparation of biological samples for analysis for stability studies. The differential pulse voltammetric (DPV) method using boron doped diamond electrode (BDDE), based on characteristic oxidation peaks, was proposed and successfully applied. The linearity range was within 5.0 × 10−6 to 5.0 × 10−5 M of brimonidine, and limit of detection and limit of quantitation were 1.94 × 10−6 M and 6.46 × 10−6 M, respectively. Intra-day and inter-day precision and accuracy were evaluated and all results were in accordance with validation ICH guidelines. The best short-term stability study results were obtained for a concentration level of 3.0 × 10−5 M expressed by deviation of + 1.86% between initial and post storage concentrations. A long-term stability study was performed for two concentrations of 3.0 × 10−5 M and 5.0 × 10−5 M and resulted in deviations of + 1.63% and + 3.56%, respectively. A freeze and thaw stability study indicated that samples might be frozen only once. The enhancement of DPV/BDDE method sensitivity gained by modification, for the analysis of immeasurable BRIM quantities in native, untreated aqueous humor, was reached for quantities of 6 or 12 nmol/0.1 mL aqueous humor with acceptable accuracy (up to + 7.5%). The nature of the process—the irreversible one electron oxidation voltammetric peak of BRIM—limited the sensitivity. Only electrochemical pre-treatment of the BDD electrode before each measurement significantly speeded up the whole procedure. The advantages of the proposed method are simplicity, short-time performance, and good specificity/selectivity, as well as satisfactory accuracy, and no chemical modification of BDDE was necessary.en
dc.publisherSpringer Verlag
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS//
dc.rightsrestrictedAccess
dc.sourceAnalytical and Bioanalytical Chemistry
dc.subjectAqueous humoren
dc.subjectBoron doped diamond electrodeen
dc.subjectBrimonidineen
dc.subjectDeterminationen
dc.subjectDifferential pulse voltammetryen
dc.subjectStability studyen
dc.titleThe evaluation of short- and long-term stability studies for brimonidine in aqueous humor by DPV/BDDE method—possible application for direct assay in native samplesen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКапетановић, Вера; Стојковић, М; Марјановић, И; Јовановић, М; Рајић, К.К; Aлексић, Мара; Aгбаба, Даница; Радуловић, Валентина;
dc.citation.rankM21
dc.identifier.wos000482452600015
dc.identifier.doi10.1007/s00216-019-01955-3
dc.identifier.scopus2-s2.0-85068035860
dc.identifier.rcubconv_4543
dc.type.versionpublishedVersion


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