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dc.creatorNovović, Katarina
dc.creatorMalesević, Milka
dc.creatorFilipić, Brankica
dc.creatorMirković, Nemanja
dc.creatorMiljković, Marija
dc.creatorKojić, Milan
dc.creatorJovčić, Branko
dc.date.accessioned2019-09-02T12:10:18Z
dc.date.available2019-09-02T12:10:18Z
dc.date.issued2019
dc.identifier.issn0343-8651
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3313
dc.description.abstractPseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, rpoS and psrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in rpoS and psrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsrestrictedAccess
dc.sourceCurrent Microbiology
dc.titlePsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКојић, Милан; Мирковић, Немања; Нововић, Катарина; Малесевић, Милка; Миљковић, Марија; Филипић, Бранкица; Јовчић, Бранко;
dc.citation.volume76
dc.citation.issue3
dc.citation.spage320
dc.citation.epage328
dc.citation.other76(3): 320-328
dc.citation.rankM23
dc.identifier.wos000459160100008
dc.identifier.doi10.1007/s00284-019-01626-7
dc.identifier.pmid30684026
dc.identifier.scopus2-s2.0-85060615703
dc.identifier.rcubconv_4322
dc.type.versionpublishedVersion


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