Приказ основних података о документу

dc.creatorBurja, Blaz
dc.creatorKuret, Tadeja
dc.creatorJanko, Tea
dc.creatorTopalović, Dijana
dc.creatorŽivković, Lada
dc.creatorMrak-Poljsak, Katjusa
dc.creatorPotparević, Biljana
dc.creatorZigon, Polona
dc.creatorDistler, Oliver
dc.creatorCucnik, Sasa
dc.creatorSodin-Semrl, Snezna
dc.creatorLakota, Katja
dc.creatorFrank-Bertoncelj, Mojca
dc.date.accessioned2019-09-02T12:11:10Z
dc.date.available2019-09-02T12:11:10Z
dc.date.issued2019
dc.identifier.issn2297-055X
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3344
dc.description.abstractOlive leaf extract (OLE) is used in traditional medicine as a food supplement and as an over-the-counter drug for a variety of its effects, including anti-inflammatory and anti-atherosclerotic ones. Mechanisms through which OLE could modulate these pathways in human vasculature remain largely unknown. Serum amyloid A (SAA) plays a causal role in atherosclerosis and cardiovascular diseases and induces pro-inflammatory and pro-adhesive responses in human coronary artery endothelial cells (HCAEC). Within this study we explored whether OLE can attenuate SAA-driven responses in HCAEC. HCAEC were treated with SAA (1,000 nM) and/or OLE (0.5 and 1 mg/ml). The expression of adhesion molecules VCAM-1 and E-selectin, matrix metalloproteinases (MMP2 and MMP9) and microRNA 146a, let-7e, and let-7g (involved in the regulation of inflammation) was determined by qPCR. The amount of secreted IL-6, IL-8, MIF, and GRO-alpha in cell culture supernatants was quantified by ELISA. Phosphorylation of NF-kappa B was assessed by Western blot and DNA damage was measured using the COMET assay. OLE decreased significantly released protein levels of IL-6 and IL-8, as well as mRNA expression of E-selectin in SAA-stimulated HCAEC and reduced MMP2 levels in unstimulated cells. Phosphorylation of NF-kappa B (p65) was upregulated in the presence of SAA, with OLE significantly attenuating this SAA-induced effect. OLE stabilized SAA-induced upregulation of microRNA-146a and let-7e in HCAEC, suggesting that OLE could fine-tune the SAA-driven activity of NF-kappa B by changing the microRNA networks in HCAEC. SAA induced DNA damage and worsened the oxidative DNA damage in HCAEC, whereas OLE protected HCAEC from SAA- and H2O2-driven DNA damage. OLE significantly attenuated certain pro-inflammatory and pro-adhesive responses and decreased DNA damage in HCAEC upon stimulation with SAA. The reversal of SAA-driven endothelial activation by OLE might contribute to its anti-inflammatory and anti-atherogenic effects in HCAEC.en
dc.publisherFrontiers Media Sa, Lausanne
dc.relationSlovenian Research Agency - BU-RS/16-17-019
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceFrontiers in Cardiovascular Medicine
dc.subjectOLEen
dc.subjectSAAen
dc.subjectHCAECen
dc.subjectinflammationen
dc.subjectatherosclerosisen
dc.subjectmicroRNAen
dc.subjectDNA damageen
dc.titleOlive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cellsen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractМрак-Пољсак, Катјуса; Зигон, Полона; Дистлер, Оливер; Бурја, Блаз; Потпаревић, Биљана; Цуцник, Саса; Живковић, Лада; Содин-Семрл, Снезна; Лакота, Катја; Топаловић, Дијана; Франк-Бертонцељ, Мојца; Курет, Тадеја; Јанко, Теа;
dc.citation.volume6
dc.citation.other6: -
dc.citation.rankM22
dc.identifier.wos000468066500001
dc.identifier.doi10.3389/fcvm.2019.00056
dc.identifier.pmid31157238
dc.identifier.scopus2-s2.0-85074985667
dc.identifier.fulltexthttps://farfar.pharmacy.bg.ac.rs//bitstream/id/1909/3342.pdf
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу