Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa
Abstract
Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin... production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100-175 mu g/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.
Keywords:
Chalcones / Acinetobacter baumannii / Pseudomonas aeruginosa / Biofilm / MotilitySource:
Microbial Pathogenesis, 2019, 131, 186-196Publisher:
- Academic Press Ltd- Elsevier Science Ltd, London
DOI: 10.1016/j.micpath.2019.04.015
ISSN: 0882-4010
PubMed: 30980878
WoS: 000471208000026
Scopus: 2-s2.0-85064077948
Collections
Institution/Community
PharmacyTY - JOUR AU - Usjak, Dušan AU - Ivković, Branka AU - Božić, Dragana AU - Bošković, Lidija AU - Milenković, Marina PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3351 AB - Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100-175 mu g/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research. PB - Academic Press Ltd- Elsevier Science Ltd, London T2 - Microbial Pathogenesis T1 - Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa VL - 131 SP - 186 EP - 196 DO - 10.1016/j.micpath.2019.04.015 ER -
@article{ author = "Usjak, Dušan and Ivković, Branka and Božić, Dragana and Bošković, Lidija and Milenković, Marina", year = "2019", abstract = "Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100-175 mu g/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.", publisher = "Academic Press Ltd- Elsevier Science Ltd, London", journal = "Microbial Pathogenesis", title = "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa", volume = "131", pages = "186-196", doi = "10.1016/j.micpath.2019.04.015" }
Usjak, D., Ivković, B., Božić, D., Bošković, L.,& Milenković, M.. (2019). Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis Academic Press Ltd- Elsevier Science Ltd, London., 131, 186-196. https://doi.org/10.1016/j.micpath.2019.04.015
Usjak D, Ivković B, Božić D, Bošković L, Milenković M. Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis. 2019;131:186-196. doi:10.1016/j.micpath.2019.04.015 .
Usjak, Dušan, Ivković, Branka, Božić, Dragana, Bošković, Lidija, Milenković, Marina, "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa" in Microbial Pathogenesis, 131 (2019):186-196, https://doi.org/10.1016/j.micpath.2019.04.015 . .