FarFaR - Farmaceutski fakultet, repozitorijum
Univerzitet u Beogradu, Farmaceutski fakultet
    • English
    • Српски
    • Српски (Serbia)
  • Srpski (latinica) 
    • Engleski
    • Srpski (ćirilica)
    • Srpski (latinica)
  • Prijava
Pregled rada 
  •   FarFaR - Farmaceutski fakultet, repozitorijum
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled rada
  •   FarFaR - Farmaceutski fakultet, repozitorijum
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • Pregled rada
JavaScript is disabled for your browser. Some features of this site may not work without it.

Telomerase stability and evaluation of real-time telomeric repeat amplification protocol

Samo za registrovane korisnike
2019
Autori
Vukašinović, Aleksandra
Kotur-Stevuljević, Jelena
Mlakar, Vid
Sopić, Miron
Cvetković, Zorica P.
Petković, Miloš
Spasojević-Kalimanovska, Vesna
Bogavac-Stanojević, Nataša
Ostanek, Barbara
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples ...obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p lt .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p lt .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.

Ključne reči:
Telomerase / freezing / reproducibility of results / biomarkers / carcinoma / nuclear magnetic resonance
Izvor:
Scandinavian Journal of Clinical and Laboratory Investigation, 2019, 79, 3, 188-193
Izdavač:
  • Taylor & Francis Ltd, Abingdon
Projekti:
  • Interaktivna uloga dislipidemije, oksidativnog stresa i inflamacije u aterosklerozi i drugim bolestima: genetički i biohemijski markeri (RS-175035)

DOI: 10.1080/00365513.2019.1576220

ISSN: 0036-5513

PubMed: 30767570

WoS: 000465174400006

Scopus: 2-s2.0-85061797797
[ Google Scholar ]
1
1
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/3356
Kolekcije
  • Radovi istraživača / Researchers’ publications
Institucija
Pharmacy

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB
 

 

Kompletan repozitorijumInstitucijeAutoriNasloviTemeOva institucijaAutoriNasloviTeme

Statistika

Pregled statistika

DSpace software copyright © 2002-2015  DuraSpace
O FarFaR-u | Pošaljite zapažanja

OpenAIRERCUB