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dc.creatorVukašinović, Aleksandra
dc.creatorKotur-Stevuljević, Jelena
dc.creatorMlakar, Vid
dc.creatorSopić, Miron
dc.creatorCvetković, Zorica P.
dc.creatorPetković, Miloš
dc.creatorSpasojević-Kalimanovska, Vesna
dc.creatorBogavac-Stanojević, Nataša
dc.creatorOstanek, Barbara
dc.date.accessioned2019-09-02T12:11:32Z
dc.date.available2019-09-02T12:11:32Z
dc.date.issued2019
dc.identifier.issn0036-5513
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3356
dc.description.abstractTelomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements. Samples used for stability and freeze-thaw study were peripheral blood leukocytes, obtained from apparently healthy persons, patients with diagnosed cancer and cell lines. Telomerase activity was measured using TRAP method, while standard evaluation was done using nuclear magnetic resonance (NMR) technique. Storage at -20 degrees C preserved telomerase activity in samples from cancer patients for at least 14 days (21.46 +/- 0.135 versus 21.84 +/- 0.357, p = .756), while samples obtained from healthy persons should be stored at -80 degrees C. We observed significant decrease of telomerase activity at freeze thaw cycle 5 in cancer patients' samples (21.46 +/- 0.135 versus 23.09 +/- 0.316, p lt .05), and in healthy persons' ones already at cycle 3 (22.74 +/- 0.107 versus 24.85 +/- 0.151, p lt .05). Telomerase activity from cell lines samples showed overall greater stability regarding the storage period and freeze-thaw cycles and it was considered for standard sample, which was confirmed by NMR analysis. Telomerase enzyme had adequate stability while efficacy, linearity, and reproducibility of TRAP method were acceptable for bio-analytical methods. All this indicated that telomerase could be a reliable biomarker.en
dc.publisherTaylor & Francis Ltd, Abingdon
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175035/RS//
dc.rightsrestrictedAccess
dc.sourceScandinavian Journal of Clinical and Laboratory Investigation
dc.subjectTelomeraseen
dc.subjectfreezingen
dc.subjectreproducibility of resultsen
dc.subjectbiomarkersen
dc.subjectcarcinomaen
dc.subjectnuclear magnetic resonanceen
dc.titleTelomerase stability and evaluation of real-time telomeric repeat amplification protocolen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПетковић, Милош; Котур-Стевуљевић, Јелена; Вукашиновић, Aлександра; Цветковић, Зорица П.; Спасојевић-Калимановска, Весна; Останек, Барбара; Богавац-Станојевић, Наташа; Сопић, Мирон; Млакар, Вид;
dc.citation.volume79
dc.citation.issue3
dc.citation.spage188
dc.citation.epage193
dc.citation.other79(3): 188-193
dc.citation.rankM23
dc.identifier.wos000465174400006
dc.identifier.doi10.1080/00365513.2019.1576220
dc.identifier.pmid30767570
dc.identifier.scopus2-s2.0-85061797797
dc.identifier.rcubconv_4357
dc.type.versionpublishedVersion


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