Modeling and optimization of bioactive compounds from chickpea seeds (Cicer arietinum L)
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The aim of study was to model and optimize the extraction of polyphenols and flavonoids with expressed antioxidant activity from chickpea seeds using a central composite design. The optimal extraction conditions were the extraction time of 145.5 min, ethanol concentration of 83.7% and liquid-to-solid ratio of 24.9 (expressed as cm(3) per g). The content of total polyphenol and flavonoid was 2.75 g gallic acid equivalent per kilogram of the dried extract and 0.147 g rutin equivalent per kilogram of the dried extract, respectively. Using 2,2-diphenyl-1-picrylhydrazyl assay, it was found that the optimal extract had the IC50 of 1.55 mg cm(-3).
Ključne reči:Chickpea / total polyphenol / total flavonoid / antioxidant activity / central composite design
Izvor:Solid State Ionics, 2019, 54, 5, 837-846
- Taylor & Francis Inc, Philadelphia