Show simple item record

dc.creatorTumpa, Anja
dc.creatorRakić, Tijana
dc.creatorJovanović, Marko
dc.creatorJančić-Stojanović, Biljana
dc.date.accessioned2019-09-14T21:49:52Z
dc.date.available2016-08-17
dc.date.issued2015
dc.identifier.issn1082-6076
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2332
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3443
dc.description.abstractThe retention behavior of substances in hydrophilic interaction liquid chromatography (HILIC) is difficult to predict. Mixture investigated in this paper consists of acetylsalicylic acid, amlodipine, impurity A of amlodipine, and atenolol, a very often used combination in treatment of some cardiovascular diseases. Retention behavior dependence on the most influential chromatographic factors is described by mathematical models, with the special emphasis on pH of the mobile phase. D-optimal design is applied to generate more complex models and to obtain more accurate results. Comparison of Predicted R-2 values of quadratic and cubic model for pH dependence (0.847 and 0.934, respectively) shows that the cubic model has significantly better prediction ability than quadratic in the investigated system. After describing retention behavior, chemometrical tools (indirect modeling of complex chromatographic responses and grid point search optimization) are used to locate the optimal conditions for analyzed mixture in terms of satisfactory separation and minimal analysis duration. The optimal conditions are Column: Kinetex HILIC 100 A (100mmx4.5mm, 2.6 mu m particle size); injection volume: 5 mu L; flow rate: 1mLmin(-1); column temperature: 30 degrees C; detection wavelength: 254nm; mobile phase: acetonitrile-water phase (75mM ammonium acetate, pH 5.3) (91:9V/V).en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsembargoedAccess
dc.sourceJournal of Liquid Chromatography & Related Technologies
dc.titleOptimization of Chromatographic Separation of Acetylsalicylic Acid, Amlodipine, Impurity a of Amlodipine, and Atenolol in Hydrophilic Interaction Liquid Chromatography Employing DoE Methodologyen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЈовановић, Марко; Ракић, Тијана; Тумпа, Aња; Јанчић-Стојановић, Биљана;
dc.citation.volume38
dc.citation.issue16
dc.citation.spage1555
dc.citation.epage1560
dc.citation.other38(16): 1555-1560
dc.citation.rankM23
dc.description.otherThis is peer-reviewed version of the following article: Tumpa, A. R.; Rakić, T. M.; Jovanović, M. M.; Jančić-Stojanović, B. S. Optimization of Chromatographic Separation of Acetylsalicylic Acid, Amlodipine, Impurity A of Amlodipine, and Atenolol in Hydrophilic Interaction Liquid Chromatography Employing DoE Methodology. J. Liq. Chromatogr. Relat. Technol. 2015, 38 (16), 1555–1560. [https://doi.org/10.1080/10826076.2015.1077863]
dc.identifier.wos000361519100001
dc.identifier.doi10.1080/10826076.2015.1077863
dc.identifier.scopus2-s2.0-84942743718
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/7179/Optimization_of_Chromatographic_acc_2015.pdf
dc.type.versionacceptedVersion
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record